CXCL10/IP-10/CRG-2 Antibody [Unconjugated]

• Reactivity: Cr
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL10/IP-10/CRG-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant cotton rat CXCL10/IP‑10/CRG‑2
  Thr27-Val94
  Accession # AAL16935
• Specificity: Detects cotton rat CXCL10/IP‑10/CRG‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CXCL-16 is observed. In Western blots, less than 5% cross‑reactivity with recombinant human CXCL10 and recombinant mouse CRG-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL10
• Purity: Immunogen affinity purified
• Endotoxin Note: <0.15 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL10/IP-10/CRG-2 Antibody [Unconjugated]

• C7
• chemokine (C-X-C motif) ligand 10
• CRG2
• CRG-2
• CXCL10
• gIP-10
• IFI10
• INP10
• IP-10
• mob-1
• SCYB10

Background

IP-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1‑7).

1. Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049.
2. Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515.
3. Proost, P. et al. (1993) J. Immunol. 150:1000.
4. Blanco, J.C. et al. (2001) GenBank Accession # AAL16959.
5. Neville, L.F. (1997) Prog. Growth Factor Res. 8:207.
6. Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1.
7. Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCL10
• Uniprot:
  • AAL16935()