CXCL10/IP-10/CRG-2 Antibody [Unconjugated]

CXCL10/IP‑10/CRG‑2 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-MouseCXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Recombinant Mouse CXCL10/CRG‑2 (Catalog # 466‑CR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/CRG‑2 (0.5 µg/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Mouse CXCL10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA). The ND50 is typically 5‑25 µg/mL.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL10/IP‑10/CRG‑2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL10/IP-10/CRG-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2
  Ile22-Pro98
  Accession # P17515
• Specificity: Detects mouse CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human CXCL10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Cxcl10
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL10/IP-10/CRG-2 Antibody [Unconjugated]

• C7
• chemokine (C-X-C motif) ligand 10
• CRG2
• CRG-2
• CXCL10
• gIP-10
• IFI10
• INP10
• IP-10
• mob-1
• SCYB10

Background

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
3. Sgadari, C. et al. (1996) Blood 87:3877.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL10/IP-10/CRG-2 Antibody (AF-466-NA)(17)

Publications using AF-466-NA

• M Chen, RR Reed, AP Lane Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense Cell Stem Cell, 2019;25(4):501-513.e5. 2019 [PMID: 31523027] (IHC-Fr, Mouse)
• S Hiyari, E Green, C Pan, S Lari, M Davar, R Davis, PM Camargo, S Tetradis, AJ Lusis, FQ Pirih Genome-Wide Association Study Identifies Cxcl Family Members as Partial Mediators of LPS-induced Periodontitis J. Bone Miner. Res., 2018;0(0):. 2018 [PMID: 29637625] (IHC-P, Mouse)
• M Muramatsu, L Gao, J Peresie, B Balderman, S Akakura, IH Gelman SSeCKS/AKAP12 scaffolding functions suppress B16F10-induced peritoneal metastasis by attenuating CXCL9/10 secretion by resident fibroblasts Oncotarget, 2017;8(41):70281-70298. 2017 [PMID: 29050279] (Neutralization, Mouse)
• VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury Sci Rep, 2017;7(0):46514. 2017 [PMID: 28425451] (Neutralization, Mouse)
• X Li, QY Yao, HC Liu, QW Jin, BL Xu, SC Zhang, CT Tu Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28378526] (IHC, Mouse)
• Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy J Neuroinflammation, 2016;13(1):298. 2016 [PMID: 27894304] (IHC-Fr, Mouse)
• CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata J Immunol, 2016;0(0):. 2016 [PMID: 27412416] (Flow Cytometry, Mouse)
• Mills Ko E, Ma J, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika A, Pleasure D Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model. J Neuroinflammation, 2014;11(0):105. 2014 [PMID: 24924222] (Western Blot, Mouse)
• Zeng X, Nagavalli A, Smith C, Howard J, Su M Divergent effects of T cell costimulation and inflammatory cytokine production on autoimmune peripheral neuropathy provoked by Aire deficiency. J Immunol, 2013;190(8):3895-904. 2013 [PMID: 23487421] (IHC, Mouse)
• Leconet W, Petit P, Peraldi-Roux S, Bresson D Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes. Mol Ther, 2012;20(12):2315-25. 2012 [PMID: 22990670] (Western Blot, Mouse)
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010;120(3):907-23. 2010 [PMID: 20197626] (Flow Cytometry, Mouse)
• King VL, Lin AY, Kristo F, Anderson TJ, Ahluwalia N, Hardy GJ, Owens AP, Howatt DA, Shen D, Tager AM, Luster AD, Daugherty A, Gerszten RE Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture. Circulation, 2009;119(3):426-35. 2009 [PMID: 19139386] (IHC-Fr, Mouse)
• Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus. J. Immunol., 2007;179(11):7949-58. 2007 [PMID: 18025243] (IHC-P, Mouse)
• Maeno T, Houghton AM, Quintero PA, Grumelli S, Owen CA, Shapiro SD CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice. J. Immunol., 2007;178(12):8090-6. 2007 [PMID: 17548647] (ICC, Mouse)
• Fujita H, Asahina A, Komine M, Tamaki K The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell. Immunol., 2007;245(2):70-9. 2007 [PMID: 17507001] (Functional Assay, Mouse)
• Bitko V, Musiyenko A, Barik S Viral infection of the lungs through the eye. J. Virol., 2007;81(2):783-90. 2007 [PMID: 17050596] (Western Blot, Mouse)
• Cardona AE, Gonzalez PA, Teale JM CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Infect. Immun., 2003;71(5):2634-42. 2003 [PMID: 12704138] (ICC, Mouse)

Bioinformatics

• Gene Symbol: Cxcl10
• Uniprot:
  • P17515()