CXCL10/IP-10/CRG-2 Antibody [Unconjugated]

CXCL10/IP-10 was detected in immersion fixed PHA-treated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CXCL10/IP‑10/CRG‑2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Human CXCL10/IP‑10/CRG‑2 protein was serially diluted 2-fold and captured by Mouse Anti-Human CXCL10/IP‑10/CRG‑2 Monoclonal Antibody (Catalog # MAB2661) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Recombinant Human CXCL10/IP‑10 (Catalog # 266-IP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL10/IP‑10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL10/IP‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA). The ND50 is typically 1-4 µg/mL.

• Reactivity: Hu
• Applications: ELISA, IHC, B/N, CyTOF-ready, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL10/IP-10/CRG-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL10/IP-10/CRG-2
  Val22-Pro98
  Accession # P02778
• Specificity: Detects human CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse CRG-2 and recombinant rat CRG-2 is observed.
• Details of Functionality: ActivityAssaywCitation not found None None None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL10
• Endotoxin Note: <0.20 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 1-4 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL10/IP-10/CRG-2 Antibody [Unconjugated]

• C7
• chemokine (C-X-C motif) ligand 10
• CRG2
• CRG-2
• CXCL10
• gIP-10
• IFI10
• INP10
• IP-10
• mob-1
• SCYB10

Background

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts, and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and MIG has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
2. Wang, X. et al.(1996) J. Biol. Chem. 271:24286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL10/IP-10/CRG-2 Antibody (AF-266-NA)(19)

Publications using AF-266-NA

• JL Booth, ES Duggan, VI Patel, W Wu, DM Burian, DC Hutchings, VL White, KM Coggeshall, MG Dozmorov, JP Metcalf Gene expression profiling of primary human type I alveolar epithelial cells exposed to Bacillus anthracis spores reveals induction of neutrophil and monocyte chemokines Microb. Pathog., 2018;0(0):. 2018 [PMID: 29704667] (Neutralization, Human)
• S Urra, MC Fischer, JR Martínez, L Véliz, P Orellana, A Solar, K Bohmwald, A Kalergis, C Riedel, AH Corvalán, JC Roa, R Fuentealba, CJ Cáceres, M López-Last, A León, N Droppelman, HE González Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis? Oncotarget, 2018;9(2):2445-2467. 2018 [PMID: 29416784] (IHC-P, Human)
• RS Dagur, W Wang, Y Cheng, E Makarov, M Ganesan, H Suemizu, CL Gebhart, S Gorantla, N Osna, LY Poluektova Human hepatocytes depletion in the presence of HIV-1 infection in dual reconstituted humanized mice Biol Open, 2018;0(0):. 2018 [PMID: 29361613] (IHC-P, Humanized Mouse)
• CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata J Immunol, 2016;0(0):. 2016 [PMID: 27412416] (IHC-P, Human)
• M Liu, X Zeng, J Wang, Z Fu, J Wang, M Liu, D Ren, B Yu, L Zheng, X Hu, W Shi, J Xu Immunomodulation by mesenchymal stem cells in treating human autoimmune disease-associated lung fibrosis Stem Cell Res Ther, 2016;7(1):63. 2016 [PMID: 27107963] (Neutralization, Human)
• Shang L, Smith A, Duan L, Perkey K, Qu L, Wietgrefe S, Zupancic M, Southern P, Masek-Hammerman K, Reeves R, Johnson R, Haase A NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques. J Immunol, 2014;193(1):277-84. 2014 [PMID: 24899503] (IHC, Primate - Macaca mulatta (Rhesus Macaque))
• Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D Expression and regulation of chemokines in murine and human type 1 diabetes. Diabetes, 2012;61(2):436-46. 2012 [PMID: 22210319] (Flow Cytometry, Human)
• Shan M, Cheng HF, Song LZ Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema. Sci Transl Med, 2009;1(4):4ra10. 2009 [PMID: 20368170] (ICC, Human)
• Maingat F, Viappiani S, Zhu Y, Vivithanaporn P, Ellestad KK, Holden J, Silva C, Power C Regulation of lentivirus neurovirulence by lipopolysaccharide conditioning: suppression of CXCL10 in the brain by IL-10. J. Immunol., 2010;184(3):1566-74. 2010 [PMID: 20042580] (IHC-P, Feline)
• Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease. J. Immunol., 2009;182(6):3919-27. 2009 [PMID: 19265171] (IHC-Fr, Human)
• Okumura S, Yuki K, Kobayashi R, Okamura S, Ohmori K, Saito H, Ra C, Okayama Y Hyperexpression of NOD2 in intestinal mast cells of Crohn&#039;s disease patients: preferential expression of inflammatory cell-recruiting molecules via NOD2 in mast cells. Clin. Immunol., 2009;130(2):175-85. 2009 [PMID: 18938111] (IHC-Fr, Human)
• van Heteren JT, Rozenberg F, Aronica E, Troost D, Lebon P, Kuijpers TW Astrocytes produce interferon-alpha and CXCL10, but not IL-6 or CXCL8, in Aicardi-Goutières syndrome. Glia, 2008;56(5):568-78. 2008 [PMID: 18240301] (IHC-P, Human)
• Datta D, Flaxenburg JA, Laxmanan S, Geehan C, Grimm M, Waaga-Gasser AM, Briscoe DM, Pal S Ras-induced modulation of CXCL10 and its receptor splice variant CXCR3-B in MDA-MB-435 and MCF-7 cells: relevance for the development of human breast cancer. Cancer Res., 2006;66(19):9509-18. 2006 [PMID: 17018607] (IHC-Fr, Human)
• Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus. Arthritis Rheum., 2005;52(5):1504-16. 2005 [PMID: 15880822] (IHC-Fr, Human)
• van Marle G, Henry S, Todoruk T, Sullivan A, Silva C, Rourke SB, Holden J, McArthur JC, Gill MJ, Power C Human immunodeficiency virus type 1 Nef protein mediates neural cell death: a neurotoxic role for IP-10. Virology, 2004;329(2):302-18. 2004 [PMID: 15518810] (ICC, Human)
• Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis. Int. J. Oncol., 2004;25(3):605-13. 2004 [PMID: 15289861] (IHC-P, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003;73(5):584-90. 2003 [PMID: 12714572] (Neutralization, Human)
• Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. J. Immunol., 2002;169(11):6515-21. 2002 [PMID: 12444162] (ELISA Development, Human)
• Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees. J. Infect. Dis., 1999;179(3):646-52. 1999 [PMID: 9952371] (ELISA Development, Primate - Pan troglodytes (Chimpanzee))

Reviews for CXCL10/IP-10/CRG-2 Antibody (AF-266-NA) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous IF Human 01/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23423575
• Species: Human

Blocking

• Blocking Details: See PMID 23423575

Details

• Detection Notes: See PMID 23423575

Comments

• Comments: Published: See PMID 23423575also worked well in cell culture experiments as functional antibody

reviewed by: Anonymous IHC-P Human 01/07/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 24080276
• Species: Human

Bioinformatics

• Gene Symbol: CXCL10
• Uniprot:
  • P02778()