CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin]

• Reactivity: Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant rat CXCL1/GRO alpha /KC/CINC‑1
  Ala25-Lys96
  Accession # P14095
• Specificity: Detects rat CXCL1/GRO alpha /KC/CINC‑1 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) KC is observed and less than 10% cross-reactivity with recombinant human (rh) GRO alpha , rhGRO beta , rhGRO gamma , and rmMIP-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Cxcl1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody [Biotin]

• CINC1
• CINC-1
• CXCL1
• FSP
• GRO alpha
• GRO1
• GROa
• KC
• MGSA
• MGSA-a
• MGSA-alpha
• NAP-3
• SCYB1

Background

Cytokine-induced neutrophil chemoattractant 1 (CINC-1) was originally purified from media conditioned by IL-1 beta stimulated rat kidney epithelioid cells (NRK-52E). On the basis of its protein and DNA sequences, CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2 alpha , CINC-2 beta , CINC-3/MIP-2), sharing approximately 63-67% amino acid sequence identity with CINC-1, have been identified. The protein sequence of rat CINC-1 is also 68%, 71%, and 69% identical to that of human GRO-alpha , GRO-beta , and GRO-gamma , respectively. Based on their sequence homology, it has been suggested that CINCs are the rat counterpart of human GROs.

Rat CINC-1 cDNA encodes a 96 amino acid residue precursor protein from which the amino-terminal 24 amino acid residues are cleaved to generate the mature CINC-1. Similar to other alpha chemokines, rat CINCs are potent neutrophil attractants and activators and have been shown to play an important role in the infiltration of neutrophils into inflammatory sites in rats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL1/GRO alpha/KC/CINC-1 Antibody (BAF515)(1)

Publication using BAF515

• Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology. Infect. Immun., 2008-06-09;76(8):3577-86. 2008-06-09 [PMID: 18541654] (ELISA Development, Rat)

Bioinformatics

• Gene Symbol: Cxcl1
• Uniprot:
  • P14095()