CXCL1/GRO alpha/KC/CINC-1 Antibody [Unconjugated]

Recombinant Rat CXCL1/CINC‑1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC‑1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rat CXCL1/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA). The ND50 is typically 0.15‑0.75 µg/mL.

CXCL1/GROa/KC/CINC‑1 was detected in perfusion fixed paraffin-embedded sections of rat liver using 15 µg/mL Rat CXCL1/GROa/KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑515‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CXCL1/GROa/KC/CINC-1 was detected in perfusion fixed frozen sections of rat liver using Rat CXCL1/GROa/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Rt
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL1/GRO alpha/KC/CINC-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat CXCL1/GRO alpha /KC/CINC-1
  Ala25-Lys96
  Accession # P14095
• Specificity: Detects rat CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse (rm) KC is observed and less than 10% cross-reactivity with recombinant human (rh) GRO beta , rhGRO gamma , rmMIP-2, recombinant rat (rr) CINC-2 alpha and rrCINC-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Cxcl1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.15-0.75 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody [Unconjugated]

• CINC1
• CINC-1
• CXCL1
• FSP
• GRO alpha
• GRO1
• GROa
• KC
• MGSA
• MGSA-a
• MGSA-alpha
• NAP-3
• SCYB1

Background

Cytokine-induced neutrophil chemoattractant 1 (CINC-1) was originally purified from media conditioned by IL-1 beta stimulated rat kidney epithelioid cells (NRK-52E). On the basis of its protein and DNA sequences, CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2 alpha , CINC-2 beta , CINC-3/MIP-2), sharing approximately 63-67% amino acid sequence identity with CINC-1, have been identified. The protein sequence of rat CINC-1 is also 68%, 71%, and 69% identical to that of human GRO-alpha , GRO-beta , and GRO-gamma , respectively. Based on their sequence homology, it has been suggested that CINCs are the rat counterpart of human GROs.

Rat CINC-1 cDNA encodes a 96 amino acid residue precursor protein from which the amino-terminal 24 amino acid residues are cleaved to generate the mature CINC-1. Similar to other alpha chemokines, rat CINCs are potent neutrophil attractants and activators and have been shown to play an important role in the infiltration of neutrophils into inflammatory sites in rats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL1/GRO alpha/KC/CINC-1 Antibody (AF-515-NA)(4)

Publications using AF-515-NA

• Kuo, CH;Lee, GH;Wu, HL;Huang, JY;Tang, MJ; Breaking the symmetry of cell contractility drives tubulogenesis via CXCL1 polarization Proceedings of the National Academy of Sciences of the United States of America 2024-02-27 [PMID: 38377213] (Immunocytochemistry, Rat)
• M. D. Shahaduzzaman, Vijay Mehta, Jason E. Golden, Derrick D. Rowe, Suzanne Green, Ramya Tadinada et al. Human Umbilical Cord Blood Cells Induce Neuroprotective Change in Gene Expression Profile in Neurons after Ischemia through Activation of Akt Pathway Cell Transplantation 2015-04-01 [PMID: 25413246]
• Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology. Infect. Immun., 2008-06-09;76(8):3577-86. 2008-06-09 [PMID: 18541654] (ELISA Development, Rat)
• Noguchi M, Yuzurihara M, Kase Y, Yasui T, Irahara M Involvement of cytokine-induced neutrophil chemoattractant in hypothalamic thermoregulation of luteinizing hormone-releasing hormone. Endocrinology, 2008-03-06;149(6):2899-906. 2008-03-06 [PMID: 18325994] (Neutralization, Rat)

Bioinformatics

• Gene Symbol: Cxcl1
• Uniprot:
  • P14095()