CXCL1/GRO alpha/KC/CINC-1 Antibody [Unconjugated]

Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL1/KC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA). The ND50 is typically 0.3‑1.5 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL1/GRO alpha/KC/CINC-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
  Arg20-Lys96
  Accession # P12850
• Specificity: Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant rat CINC-1 is observed and less than 5% cross-reactivity with recombinant mouse (rm) MIP-2, recombinant human (rh) IL‑8, rhGRO alpha, rhGRO beta and rmCXCL3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Cxcl1
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Neutralization 0.3-1.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody [Unconjugated]

• CINC1
• CINC-1
• CXCL1
• GRO alpha
• KC
• MGSA-alpha

Background

KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL1/GRO alpha/KC/CINC-1 Antibody (AF-453-NA)(18)

Publications using AF-453-NA

• JS Stoolman, PC Duncker, AK Huber, DA Giles, JM Washnock-S, AM Soulika, BM Segal An IFN?/CXCL2 regulatory pathway determines lesion localization during EAE J Neuroinflammation, 2018;15(1):208. 2018 [PMID: 30012158] (IHC, Mouse)
• Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses Sci Rep, 2016;6(0):37695. 2016 [PMID: 27883091] (Bioassay, Mouse)
• Marro B, Grist J, Lane T Inducible Expression of CXCL1 within the Central Nervous System Amplifies Viral-Induced Demyelination. J Immunol, 2016;196(4):1855-64. 2016 [PMID: 26773148] (IHC, Mouse)
• Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. J Biol Chem, 2015;290(4):2053-68. 2015 [PMID: 25505239] (IHC, Mouse)
• Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta. Int J Cancer, 2014;134(6):1346-58. 2014 [PMID: 24154944] (IHC-Fr, Mouse)
• Habibzay, M, Saldana, J I, Goulding, J, Lloyd, C M, Hussell, T Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung. Mucosal Immunol, 2012;5(5):524-34. 2012 [PMID: 22549744] (IHC-P, Mouse)
• Li Z, Burns AR, Miller SB, Smith CW CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing. FASEB J., 2011;25(8):2659-68. 2011 [PMID: 21518851] (Neutralization, Mouse)
• Summers SA, van der Veen BS, O'Sullivan KM Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis. Kidney Int., 2010;78(12):1263-74. 2010 [PMID: 20844472] (IHC-P, Mouse)
• Tilahun AY, Theuer JE, Patel R Detrimental effect of the proteasome inhibitor, bortezomib in bacterial superantigen- and lipopolysaccharide-induced systemic inflammation. Mol. Ther., 2010;18(6):1143-54. 2010 [PMID: 20372109] (Neutralization, Mouse)
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010;120(3):907-23. 2010 [PMID: 20197626] (Flow Cytometry, Mouse)
• Zarbock A, Distasi MR, Smith E, Sanders JM, Kronke G, Harry BL, von Vietinghoff S, Buscher K, Nadler JL, Ley K Improved survival and reduced vascular permeability by eliminating or blocking 12/15-lipoxygenase in mouse models of acute lung injury (ALI). J. Immunol., 2009;183(7):4715-22. 2009 [PMID: 19752233] (Neutralization, Mouse)
• Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9. 2009 [PMID: 19289819] (Neutralization, Mouse)
• Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1. Am. J. Pathol., 2009;174(1):164-76. 2009 [PMID: 19095949] (IHC-Fr, Mouse)
• Wu K, Katiyar S, Li A, Liu M, Ju X, Popov VM, Jiao X, Lisanti MP, Casola A, Pestell RG Dachshund inhibits oncogene-induced breast cancer cellular migration and invasion through suppression of interleukin-8. Proc. Natl. Acad. Sci. U.S.A., 2008;105(19):6924-9. 2008 [PMID: 18467491] (Neutralization, Mouse)
• Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S High pressure promotes monocyte adhesion to the vascular wall. Circ. Res., 2007;100(8):1226-33. 2007 [PMID: 17395876] (IHC, Mouse)
• Wislez M, Fujimoto N, Izzo JG, Hanna AE, Cody DD, Langley RR, Tang H, Burdick MD, Sato M, Minna JD, Mao L, Wistuba I, Strieter RM, Kurie JM High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras. Cancer Res., 2006;66(8):4198-207. 2006 [PMID: 16618742] (Western Blot, Mouse)
• Barksby HE, Hui W, Wappler I, Peters HH, Milner JM, Richards CD, Cawston TE, Rowan AD Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair. Arthritis Rheum., 2006;54(2):540-50. 2006 [PMID: 16447230] (IHC-P, Mouse)
• Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium. J. Clin. Invest., 2001;108(9):1307-14. 2001 [PMID: 11696575] (ICC, Mouse)

Bioinformatics

• Gene Symbol: Cxcl1
• Uniprot:
  • P12850()