CXCL1/GRO alpha/KC/CINC-1 Antibody

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Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CXCL1/GRO alpha/KC/CINC-1 Antibody Summary

Immunogen
E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
Arg20-Lys96
Accession # P12850
Specificity
Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rat (rr) CINC-1 is observed, 10% cross-reactivity with recombinant mouse (rm) MIP-2 and recombinant human (rh) IL‑8 is observed, and less than 5% cross-reactivity with rhGRO alpha, rhGRO beta, rmCXCL3, rrCINC-2 alpha, and rrCINC-2 beta is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Cxcl1
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 0.3-1.5 ug/mL
Publications
Read Publications using
AF-453-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody

  • CINC1
  • CINC-1
  • CXCL1
  • GRO alpha
  • KC
  • MGSA-alpha

Background

KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL1/GRO alpha/KC/CINC-1 Antibody (AF-453-NA)(18)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 10 applications: Bioassay, Flow, ICC, IHC, IHC Fluorescence, IHC Frozen, IHC Paraffin-embedded, IHC- Frozen, In vivo, WB.


Filter By Application
Bioassay
(1)
Flow
(1)
ICC
(1)
IHC
(2)
IHC Fluorescence
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(3)
IHC- Frozen
(1)
In vivo
(6)
WB
(1)
All Applications
Filter By Species
Mouse
(18)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF-453-NA Applications Species
Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses Sci Rep, 2016;6(0):37695. 2016 [PMID: 27883091] (In vivo,, Mouse) In vivo Mouse
Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses Sci Rep, 2016;6(0):37695. 2016 [PMID: 27883091] (Bioassay, Mouse) Bioassay Mouse
Marro B, Grist J, Lane T Inducible Expression of CXCL1 within the Central Nervous System Amplifies Viral-Induced Demyelination. J Immunol, 2016;196(4):1855-64. 2016 [PMID: 26773148] (IHC Fluorescence,, Mouse) IHC Fluorescence Mouse
Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. J Biol Chem, 2015;290(4):2053-68. 2015 [PMID: 25505239] (IHC,, Mouse) IHC Mouse
Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta. Int J Cancer, 2014;134(6):1346-58. 2014 [PMID: 24154944] (IHC- Frozen,, Mouse) IHC- Frozen Mouse
Habibzay, M, Saldana, J I, Goulding, J, Lloyd, C M, Hussell, T Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung. Mucosal Immunol, 2012;5(5):524-34. 2012 [PMID: 22549744] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Li Z, Burns AR, Miller SB, Smith CW CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing. FASEB J., 2011;25(8):2659-68. 2011 [PMID: 21518851] (In vivo,, Mouse) In vivo Mouse
Summers SA, van der Veen BS, O'Sullivan KM Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis. Kidney Int., 2010;78(12):1263-74. 2010 [PMID: 20844472] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Tilahun AY, Theuer JE, Patel R Detrimental effect of the proteasome inhibitor, bortezomib in bacterial superantigen- and lipopolysaccharide-induced systemic inflammation. Mol. Ther., 2010;18(6):1143-54. 2010 [PMID: 20372109] (In vivo,, Mouse) In vivo Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010;120(3):907-23. 2010 [PMID: 20197626] (Flow,, Mouse) Flow Mouse
Show All 18 Publications.

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Bioinformatics

Gene Symbol Cxcl1
Entrez
Uniprot