CX3CL1/Fractalkine Antibody [Unconjugated]

Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472). The ND50 is typically 0.3-1.5 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CX3CL1/Fractalkine Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CX3CL1/Fractalkine
  Leu22-Lys105
  Accession # O35188
• Specificity: Detects mouse Fractalkine in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Fractalkine and recombinant rat Fractalkine, 20% cross-reactivity with rhMCP-1, and 10% cross-reactivity with rhEotaxin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CX3CL1
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Neutralization 0.3-1.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody [Unconjugated]

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CL1/Fractalkine Antibody (AF472)(9)

Publications using AF472

• M Bolós, M Llorens-Ma, JR Perea, J Jurado-Arj, A Rábano, F Hernández, J Avila Absence of CX3CR1 impairs the internalization of Tau by microglia Mol Neurodegener, 2017;12(1):59. 2017 [PMID: 28810892]
• Bryan Simons PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen J. Immunol, 2016;0(0):. 2016 [PMID: 27895167] (IHC-P, Mouse)
• Yoh Goto CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow Stem Cells, 2016;0(0):. 2016 [PMID: 27339271] (Neutralization, Mouse)
• Morimura S, Sugaya M, Sato S Interaction between CX3CL1 and CX3CR1 regulates vasculitis induced by immune complex deposition. Am J Pathol, 2013;182(5):1640-7. 2013 [PMID: 23470165] (IHC, Mouse)
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010;120(3):907-23. 2010 [PMID: 20197626] (Flow Cytometry, Mouse)
• Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis. J. Immunol., 2008;181(6):4208-18. 2008 [PMID: 18768878] (ICC, Mouse)
• Ishida Y, Gao JL, Murphy PM Chemokine receptor CX3CR1 mediates skin wound healing by promoting macrophage and fibroblast accumulation and function. J. Immunol., 2008;180(1):569-79. 2008 [PMID: 18097059] (IHC, Mouse)
• Ren T, Chen Q, Tian Z, Wei H Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice. Biochem. Biophys. Res. Commun., 2007;364(4):978-84. 2007 [PMID: 17971299] (Western Blot, Mouse)
• Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects. J. Leukoc. Biol., 2007;81(1):176-85. 2007 [PMID: 17053165] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CX3CL1
• Uniprot:
  • O35188()