CX3CL1/Fractalkine Antibody (51637) [Unconjugated]

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 51637
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CX3CL1/Fractalkine Antibody (51637) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CX3CL1/Fractalkine Chemokine Domain
  Gln25-Arg339 (Ser199Asn) (predicted)
  Accession # P78423
• Specificity: Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CX3CL1 or recombinant rat CX3CL1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CX3CL1
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (51637) [Unconjugated]

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

1. Pan, Y. et al. (1997) Nature 387:611.
2. Bazan, J.F. et al. (1997) Nature 385:640.
3. Mackay, C.R. (1997) Current Biology 7:R384.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CL1/Fractalkine Antibody (MAB365)(8)

Publications using MAB365

• A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion Mediators Inflamm., 2017;2017(0):1515389. 2017 [PMID: 28522896] (Functional Assay, Human)
• Suzuki F, Kubota T Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity. Arthritis Res. Ther., 2012;14(2):R48. 2012 [PMID: 22394569] (IHC-Fr, Human)
• Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis. Blood, 2008;112(13):5026-36. 2008 [PMID: 18799722] (Neutralization, Human)
• Dame TM, Orenzoff BL, Palmer LE, Furie MB IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation. J. Immunol., 2007;178(2):1172-9. 2007 [PMID: 17202382] (Cell-based ELISA, Human)
• Faure V, Cerini C, Paul P, Berland Y, Dignat-George F, Brunet P The uremic solute p-cresol decreases leukocyte transendothelial migration in vitro. Int. Immunol., 2006;18(10):1453-9. 2006 [PMID: 16954166] (Flow Cytometry, Human)
• Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation. Eur. J. Neurosci., 2004;20(12):3222-32. 2004 [PMID: 15610155] (ICC, Human)
• Chazaud B, Sonnet C, Lafuste P, Bassez G, Rimaniol AC, Poron F, Authier FJ, Dreyfus PA, Gherardi RK Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth. J. Cell Biol., 2003;163(5):1133-43. 2003 [PMID: 14662751] (ICC, Human)
• Lucas AD, Chadwick N, Warren BF, Jewell DP, Gordon S, Powrie F, Greaves DR The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. Am. J. Pathol., 2001;158(3):855-66. 2001 [PMID: 11238035] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CX3CL1
• Uniprot:
  • P78423()