CX3CL1/Fractalkine Antibody (51637) [Allophycocyanin]

NS0 mouse myeloma cell line transfected with His-tagged human CX3CL1 was stained with Mouse Anti-His Tag PE-conjugated Monoclonal Antibody (Catalog # IC050P) and either (A) Mouse Anti-Human CX3CL1/Fractalkine APC-conjugated Monoclonal Antibody (Catalog # IC365A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 51637
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CX3CL1/Fractalkine Antibody (51637) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CX3CL1/Fractalkine Chemokine Domain
  Gln25-Arg339 (Ser199Asn) (predicted)
  Accession # P78423
• Specificity: Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CX3CL1 or recombinant rat CX3CL1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CX3CL1
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (51637) [Allophycocyanin]

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also known as Fractalkine and Neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

1. Pan, Y. et al. (1997) Nature 387:611.
2. Bazan, J.F. et al. (1997) Nature 385:640.
3. Mackay, C.R. (1997) Current Biology 7:R384.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CL1/Fractalkine Antibody (IC365A)(1)

Publication using IC365A

• S Oggero, C Cecconello, R Silva, L Zeboudj, G Sideris-La, M Perretti, M Malcangio Dorsal Root Ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain Brain, Behavior, and Immunity, 2022-09-14;106(0):289-306. 2022-09-14 [PMID: 36115544] (ICC, Human)

Bioinformatics

• Gene Symbol: CX3CL1
• Uniprot:
  • P78423()