CX3CL1/Fractalkine Antibody (126315) [Unconjugated]

Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571). The ND50 is typically 0.75‑3.5 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 126315
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CX3CL1/Fractalkine Antibody (126315) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine
• Specificity: Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1, and 50% cross-reactivity with recombinant rat CX3CL1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CX3CL1
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.75-3.5 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Mouse CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog number BAF472)
  Standard: Recombinant Mouse CX3CL1/Fractalkine Full Length (Catalog number 472-FF)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (126315) [Unconjugated]

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CL1/Fractalkine Antibody (MAB571)(5)

Publications using MAB571

• M Mizoguchi, Y Ishida, M Nosaka, A Kimura, Y Kuninaka, T Yahata, S Nanjo, S Toujima, S Minami, K Ino, N Mukaida, T Kondo Prevention of lipopolysaccharide-induced preterm labor by the lack of CX3CL1-CX3CR1 interaction in mice PLoS ONE, 2018;13(11):e0207085. 2018 [PMID: 30399192] (Neutralization, Mouse)
• A Okuma, A Hanyu, S Watanabe, E Hara p16Ink4a and p21Cip1/Waf1 promote tumour growth by enhancing myeloid-derived suppressor cells chemotaxis Nat Commun, 2017;8(1):2050. 2017 [PMID: 29234059] (In Vivo, Mouse)
• Bryan Simons PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen J. Immunol, 2016;0(0):. 2016 [PMID: 27895167] (Neutralization, Mouse)
• Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis. J Neuroinflammation, 2012;9(0):193. 2012 [PMID: 22883932] (Neutralization, Mouse)
• Tang L, Hu HD, Hu P, Lan YH, Peng ML, Chen M, Ren H Gene therapy with CX3CL1/Fractalkine induces antitumor immunity to regress effectively mouse hepatocellular carcinoma. Gene Ther., 2007;14(16):1226-34. 2007 [PMID: 17597794] (ICC, Mouse)

Bioinformatics

• Gene Symbol: CX3CL1