CWC22 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CWC22 Antibody [NBP1-84864] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.

Immunohistochemistry-Paraffin: CWC22 Antibody [NBP1-84864] - Staining of human colon shows strong nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CWC22 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSRGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CWC22
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CWC22 Protein (NBP1-84864PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CWC22 Antibody - BSA Free

• CWC22 spliceosome-associated protein homolog (S. cerevisiae)
• EIF4GL
• fSAPbpre-mRNA-splicing factor CWC22 homolog
• KIAA1604NCMfunctional spliceosome-associated protein b
• Nucampholin homolog

Background

CWC22, also known as Pre-mRNA-splicing factor CWC22 homolog, consists of a long 908 amino acid isoform that is 105 kDa, and is involved in pre-mRNA splicing, though its exact function within the process is still a focus of research. Current disease research is being conducted on the relation between CWC22 and nevus, cerebral malaria, cerebritis, and malaria. The protein is linked to a RNA metabolic processing and mRNA splicing, through which it interacts with DHX15, PRPF6, EIF4A3, FAM32A, and FRG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CWC22