Cullin 4a Antibody - N-terminal

Images

 
Western Blot: Cullin 4a Antibody [NBP1-78040] - Western Blot of Cullin 4a antibody. Lane 1: Opal Pre-Stained Ladder. Lane 2: human Testis. Lane 3: human skeletal muscle. Load: 35ug lysate per lane. Primary Antibody: ...read more
Immunohistochemistry: Cullin 4a Antibody [NBP1-78040] - Immunohistochemistry. Cullin 4a antibody was diluted 1:500 to detect Cullin 4a in human testes tissue. Tissue was formalin fixed and paraffin embedded. No ...read more
N/A: Cullin 4a Antibody [NBP1-78040]
Western Blot of Rabbit Cullin 4a Antibody - N-terminal. Lane 1: Opal Pre-Stained Ladder

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cullin 4a Antibody - N-terminal Summary

Description
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
This Cullin 4a Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of Human Cullin 4a coupled to KLH. (Uniprot: Q13619)
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
CUL4A
Purity
Delipidation and Defibrination
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:500
  • Western Blot 1:500-1:1000
Application Notes
This product has been tested by western blot, immunoprecipitation, and immunohistochemistry. The antibody immunoprecipitates protein from cell lysates (using HeLa, U2OS and others). This antibody also co-immunoprecipitates at least 8 subunits of COP9, DDB1 and CAND1. An 87.6 kDa band corresponding to human Cul4A is detected. Most cell lines expressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications.
Publications
Read Publication using NBP1-78040.

Reactivity Notes

This product reacts with human Cullin 4a. Cross reactivity is expected against mouse Cul4A based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for Cullin 4a Antibody - N-terminal

  • CUL4A
  • CUL-4A
  • Cullin 4a
  • cullin-4A

Background

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CUL4A
Uniprot