CUGBP2 Antibody

Western Blot: CUGBP2 Antibody [NBP2-16034] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: CUGBP2 Antibody [NBP2-16034] - CUGBP2 antibody detects CUGBP2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CUGBP2 antibody diluted at 1:500.

Western Blot: CUGBP2 Antibody [NBP2-16034] - Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: CUGBP2 Antibody [NBP2-16034] - Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using antibody at 1:500 dilution.

CUGBP2 antibody detects CUGBP2 protein at nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CUGBP2 protein stained by CUGBP2 antibody (NBP2-16034) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CUGBP2 Antibody Summary

• Description: Novus Biologicals Rabbit CUGBP2 Antibody (NBP2-16034) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CUGBP2. The exact sequence is proprietary.
• Localization: Nucleus; Cytoplasm
• Predicted Species: Xenopus (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CELF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CUGBP2 Antibody

• BRUNOL3ELAV-type RNA-binding protein 3
• Bruno-like protein 3
• CELF-2
• CUG triplet repeat RNA-binding protein 2
• CUG triplet repeat, RNA binding protein 2
• CUG triplet repeat, RNA-binding protein 2
• CUG-BP- and ETR-3-like factor 2
• CUGBP Elav-like family member 2
• CUGBP, Elav-like family member 2
• CUG-BP2
• CUGBP2hNAPOR
• Etr-3
• ETR3RNA-binding protein BRUNOL-3
• NAPOR
• NAPOR-2
• Neuroblastoma apoptosis-related RNA-binding protein

Background

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CELF2
• Uniprot:
  • O95319(Human)