Ctip1 Antibody

Images

 
Western Blot: Ctip1 Antibody [NB600-258] - Whole cell lysate (5, 15 and 50 ug) from Ramos cells, probed with Ctip1 Antibody diluted at 0.4 ug/ml.
Immunoprecipitation: Ctip1 Antibody [NB600-258] - Whole cell lysate (20% of IP loaded) from Ramos cells, immunoprecipitated by (NB600-258-1) at 3 ug/mg lysate and detected at a dilution of 1 ug/ml. Ctip1 was also ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Ctip1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL11A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunoprecipitation 1-5 ug/mg lysate
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB600-258 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ctip1 Antibody

  • B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
  • B-cell CLL/lymphoma 11A (zinc finger protein)
  • B-cell CLL/lymphoma 11A
  • B-cell lymphoma/leukemia 11A
  • BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
  • BCL-11A
  • BCL11A-S
  • BCL11A-XL
  • C2H2-type zinc finger protein
  • COUP-TF-interacting protein 1
  • CTIP1ecotropic viral integration site 9
  • ecotropic viral integration site 9 homolog
  • Ecotropic viral integration site 9 protein homolog
  • EVI-9
  • EVI9FLJ34997
  • HBFQTL5
  • KIAA1809BCL11A-L
  • Zinc finger protein 856
  • ZNF856FLJ10173

Background

The B cell leukemia 11A protein (BCL11A/Evi9/CTIP1) has been implicated in hematopoietic cell development and malignancies. BCL11A is a transcriptional repressor that binds directly to a GC-rich motif and is also recruited to a promoter template via interaction with the orphan nuclear receptor, chicken ovalbumin upstream promoter transcription factor II. In both cases, BCL11A-mediated transcriptional repression is only minimally reversed by trichostatin A, suggesting the possible lack of involvement of class I or II histone deacetylases. Nonetheless, chromatin immunoprecipitation assays revealed that expression of BCL11A in mammalian cells resulted in deacetylation of histones H3 and/or H4 that were associated with the promoter region of a reporter gene. BCL11A mediated transcriptional repression, as well as deacetylation of histone H3/H4 in BCL11A-transfected cells, was partially reversed by nicotinamide, an inhibitor of class III histone deacetylases such as SIRT1. SIRT1 was found to interact directly with BCL11A and was recruited to the promoter template in a BCL11A-dependent manner leading to transcriptional repression. These findings indicate a role for SIRT1 in transcriptional repression mediated by BCL11A in mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2600
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-14081
Species: Hu
Applications: IHC, IHC-P
NBP2-37380
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready
NBP1-42827
Species: Hu, Mu, Rt, Ch, Fi, Re, Xp
Applications: WB, ICC/IF, IHC, IHC-Fr
NB600-922
Species: Ch, Vi
Applications: WB, ELISA, IHC, IHC-P, IP
MAB17791
Species: Hu, Mu
Applications: WB
NBP1-51641
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-31369
Species: Hu
Applications: WB, IHC, IHC-P
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NBP1-80306
Species: Hu
Applications: WB, IHC, IHC-P
AF844
Species: Mu
Applications: WB, Block
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB500-171
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NBP2-42210
Species: Hu
Applications: WB, Flow, IHC, IHC-P
PP-H6506-00
Species: Hu
Applications: WB, IP, DirELISA
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB600-258
Species: Hu
Applications: WB, IP

Publications for Ctip1 Antibody (NB600-258)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Ctip1 Antibody (NB600-258) (0)

There are no reviews for Ctip1 Antibody (NB600-258). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ctip1 Antibody (NB600-258). (Showing 1 - 1 of 1 FAQ).

  1. Does Ctip1 Ab (NB600-258) recognizes all isoforms of the BCL11a (S,L,XL)? why is the Band size showing 117 kDa instead of 91 kDa?
    • NB600-258 was raised against the first 50 residues of Ctip1 which based on uniron is a conserved and unaltered region in all isoforms of the protein. However, we have not tested nor confirmed that this antibody recognizes any isoforms other than the canonical sequence. Also, a differential size of the protein on Western blot from what is calculated can often be attributed to post-translational modifications.

Secondary Antibodies

 

Isotype Controls

Additional Ctip1 Products

Bioinformatics Tool for Ctip1 Antibody (NB600-258)

Discover related pathways, diseases and genes to Ctip1 Antibody (NB600-258). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ctip1 Antibody (NB600-258)

Discover more about diseases related to Ctip1 Antibody (NB600-258).
 

Pathways for Ctip1 Antibody (NB600-258)

View related products by pathway.

PTMs for Ctip1 Antibody (NB600-258)

Learn more about PTMs related to Ctip1 Antibody (NB600-258).
 

Research Areas for Ctip1 Antibody (NB600-258)

Find related products by research area.

Blogs on Ctip1

There are no specific blogs for Ctip1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ctip1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol BCL11A
Entrez
Uniprot