Ctip1 Antibody (PCRP-BCL11A-1H3)

Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (PCRP-BCL11A-1H3) [NBP3-13738] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained using Ctip1 Antibody (PCRP-BCL11A-1H3) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: Ctip1 Antibody (PCRP-BCL11A-1H3) [NBP3-13738] - Flow cytometric analysis of PFA-fixed Raji cells. Ctip1 antibody (PCRP-BCL11A-1H3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometry: Ctip1 Antibody (PCRP-BCL11A-1H3) [NBP3-13738] - Flow cytometric analysis of PFA-fixed HeLa cells. Ctip1 antibody (PCRP-BCL11A-1H3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: Ctip1 Antibody (PCRP-BCL11A-1H3) [NBP3-13738] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ctip1 Antibody (PCRP-BCL11A-1H3).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-BCL11A-1H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Ctip1 Antibody (PCRP-BCL11A-1H3) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14141)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human Ctip1 protein (Uniprot: Q9H165)
• Localization: Cytoplasm, Nucleus. Associates with the nuclear body, Colocalizes with SUMO1 and SENP2 in nuclear speckles
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCL11A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 91.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ctip1 Antibody (PCRP-BCL11A-1H3)

• B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
• B-cell CLL/lymphoma 11A (zinc finger protein)
• B-cell CLL/lymphoma 11A
• B-cell lymphoma/leukemia 11A
• BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
• BCL-11A
• BCL11A-S
• BCL11A-XL
• C2H2-type zinc finger protein
• COUP-TF-interacting protein 1
• CTIP1ecotropic viral integration site 9
• ecotropic viral integration site 9 homolog
• Ecotropic viral integration site 9 protein homolog
• EVI-9
• EVI9FLJ34997
• HBFQTL5
• KIAA1809BCL11A-L
• Zinc finger protein 856
• ZNF856FLJ10173

Background

The Ctip1 gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, throug

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL11A
• Uniprot:
  • Q9H165(Human)