Ctip1 Antibody (BLR073G) - BSA Free

Western Blot: Ctip1 Antibody (BLR073G) [NBP2-78710] - Whole cell lysate (50 ug) from BW5147.3, A20, RenCa, EL4, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl11a recombinant monoclonal antibody [BLR073G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.

Flow Cytometry: Ctip1 Antibody (BLR073G) [NBP2-78710] - Rabbit anti-Bcl11a recombinant monoclonal [BLR073G] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

Western Blot: Ctip1 Antibody (BLR073G) [NBP2-78710] - Whole cell lysate (50 ug) from Ramos, HEK293T, Jurkat, MOLT-4, U2OS, and Raji cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl11a recombinantmonoclonal antibody [BLR073G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: Ctip1 Antibody (BLR073G) [NBP2-78710] - Detection of human Bcl11a by WB of immunoprecipitates from Ramos lysate using this antibody and rabbit anti-Bcl11a antibodies (NB600-258, NB600-259, NB600-261).

• Reactivity: Hu, Mu
• Applications: WB, Flow, IP
• Clone: BLR073G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ctip1 Antibody (BLR073G) - BSA Free Summary

• Description: Novus Biologicals Rabbit Ctip1 Antibody (BLR073G) - BSA Free (NBP2-78710) is a recombinant monoclonal antibody validated for use in WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A peptide representing a region between residue 1 and 50.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BCL11A
• Purity: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoprecipitation 6 uL/1 mg lysate
  • Western Blot 1:1000
• Application Notes: Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Flow Cytometry Fixed and permeabilized cells were stained with 0.2 ul per 1 x 10^6 cells.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2.
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified from cell culture supernatant

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for Ctip1 Antibody (BLR073G) - BSA Free

• B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
• B-cell CLL/lymphoma 11A (zinc finger protein)
• B-cell CLL/lymphoma 11A
• B-cell lymphoma/leukemia 11A
• BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
• BCL-11A
• BCL11A-S
• BCL11A-XL
• C2H2-type zinc finger protein
• COUP-TF-interacting protein 1
• CTIP1ecotropic viral integration site 9
• ecotropic viral integration site 9 homolog
• Ecotropic viral integration site 9 protein homolog
• EVI-9
• EVI9FLJ34997
• HBFQTL5
• KIAA1809BCL11A-L
• Zinc finger protein 856
• ZNF856FLJ10173

Background

The Ctip1 gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, throug

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL11A