CTCF Antibody [Unconjugated]

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CTCF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6369) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTCF at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CTCF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CTCF
  Met1-Ile154
  Accession # P49711
• Specificity: Detects human CTCF in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CTCF
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CTCF Antibody [Unconjugated]

• 11 zinc finger transcriptional repressor
• 11-zinc finger protein
• 11-Zinc Finger Protein2
• CCCTC-binding factor (zinc finger protein)
• CCCTC-binding Factor
• CTCF
• CTCFL paralog
• transcriptional repressor CTCF

Background

CTCF (CCCTC-binding factor) is a 130‑140 kDa member of the CTCF Zn-finger protein family. Although its predicted MW is 83 kDa, it runs anomalously slow in SDS‑PAGE. CTCF is ubiquitously expressed, save for primary spermatocytes, and serves multiple functions. It regulates both gene activation and repression by binding to unmethylated DNA sequences. Structurally, it contains eleven Zn-finger motifs, some or all of which may be involved in DNA binding. This variation in number allows for different conformations and multiple interaction partners. Human CTCF is 727 amino acids (aa) in length. It contains two SUMOylation sites (Lys74 and Lys689) and eleven consecutive C2H2-type Zn-finger domains (aa 266‑577). There are multiple Ser/Thr phosphorylation sites. Two potential alternative start sites exist; one nine aa upstream of the standard start site, and another at Met570. A third splice variant shows a 62 aa substitution for aa 349‑727. Over aa 1‑154, human CTCF shares 99% aa identity with mouse CTCF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTCF
• Uniprot:
  • P49711(Human)