CRTAC1 Antibody [Unconjugated]

Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRTAC1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of human cartilage tissue, loaded at 0.5 mg/mL. A specific band was detected for CRTAC1 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CRTAC1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CRTAC1 Isoform 1
  Ser28-Cys661
  Accession # Q9NQ79
• Specificity: Detects human CRTAC1 Isoform 1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human CRTAC1 Isoform 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CRTAC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRTAC1 Antibody [Unconjugated]

• ASPIC
• ASPIC1
• ASPIC1CEP-68acidic secreted protein in cartilage
• cartilage acidic protein 1
• CEP68
• chondrocyte expressed protein 68 kDa CEP-68,68 kDa chondrocyte-expressed protein
• CRTAC1
• FLJ10320

Background

CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661. Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRTAC1 Antibody (AF5234)(1)

Publication using AF5234

• K Takahashi, H Takeuchi, Y Kurihara, H Doi, M Kunii, K Tanaka, H Nakamura, R Fukai, A Tomita-Kat, M Tada, Y Higashiyam, H Joki, S Koyano, K Takei, F Tanaka Cerebrospinal fluid level of Nogo receptor 1 antagonist lateral olfactory tract usher substance (LOTUS) correlates inversely with the extent of neuroinflammation J Neuroinflammation, 2018-02-17;15(1):46. 2018-02-17 [PMID: 29454354] (Western Blot, Human, Mouse)

Bioinformatics

• Gene Symbol: CRTAC1
• Uniprot:
  • Q9NQ79()