CROCC Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining in human fallopian tube and liver tissues using NBP1-80820 antibody. Corresponding CROCC RNA-seq data are presented for ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human bronchus, fallopian tube, liver and testis using Anti-CROCC antibody NBP1-80820 (A) shows similar protein ...read more
Western Blot: CROCC Antibody [NBP1-80820] - Analysis in human cell line SCLC-21H.
Immunocytochemistry/ Immunofluorescence: CROCC Antibody [NBP1-80820] - Staining of human cell line U-2 OS shows localization to plasma membrane, centrosome & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human bronchus shows strong positivity in apical membrane in respiratory epithelial cells.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human testis shows very weak cytoplasmic positivity in Leydig cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CROCC Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VKRLEKQNLEKDQVNKDLTEKLEALESLRLQEQAALETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADASNGS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CROCC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. ICC/IF, WB reactivity reported (PIMD: 28100636).
Control Peptide
CROCC Protein (NBP1-80820PEP)
Publications
Read Publications using
NBP1-80820 in the following applications:

  • 4 publications
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22824794)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CROCC Antibody

  • CROCC ciliary rootlet coiled-coil, rootletin
  • ROLT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CROCC Antibody (NBP1-80820)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(4)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-80820 Applications Species
Chu Z, Gruss OJ Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells Cells Apr 1 2022 [PMID: 35406752] (ICC/IF, Human) ICC/IF Human
Mahen R. Stable centrosomal roots disentangle to allow interphase centriole independence. bioRxiv. [PMID: 29649211] (ICC/IF, Human) ICC/IF Human
Flanagan AM, Stavenschi E, Basavaraju S et al. Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification. Mol Biol Cell 2017 Mar 15 [PMID: 28100636] (WB) WB
Wallmeier J, Al-Mutairi DA, Chen CT et al. Mutations in CCNO result in congenital mucociliary clearance disorder with reduced generation of multiple motile cilia. Nat Genet 2014 Jun [PMID: 24747639]
Rohena CC, Peng J, Johnson TA et al. Chemically Diverse Microtubule Stabilizing Agents Initiate Distinct Mitotic Defects and Dysregulated Expression of Key Mitotic Kinases. Biochem Pharmacol 2013 Feb 8 [PMID: 23399639] (ICC/IF, Human) ICC/IF Human
Loffler H, Fechter A, Liu FY et al. DNA damage-induced centrosome amplification occurs via excessive formation of centriolar satellites. Oncogene 2013 Jun 13 [PMID: 22824794]
Park HS, Pich C, Chiticariu E, Bachmann D ARP-T1 is a ciliary protein associated with a novel ciliopathy in inherited basal cell cancer, Bazex-DuprE-Christol Syndrome bioRxiv Dec 16 2019 (ICC/IF, Human) ICC/IF Human
Park HS, Papanastasi E, Blanchard G et al. ARP-T1-associated Bazex-DuprE-Christol syndrome is an inherited basal cell cancer with ciliary defects characteristic of ciliopathies Communications biology May 10 2021 [PMID: 33972689] (IHC, Human) IHC Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CROCC
Entrez