CrkL [p Tyr207] Antibody

Western Blot: CrkL [p Tyr207] Antibody [NBP3-23257] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt, Ch, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CrkL [p Tyr207] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-Crk (Tyr-207) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-207 in human CrkL. This peptide sequence is well conserved in mouse and rat CrkL, and has 40% homology with the conserved site in Crk II (Tyr-221). The site is not found in Crk I. [UniProt# P46109]
• Modification: p Tyr207
• Specificity: The antibody detects a 38 kDa protein corresponding to the molecular mass of CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRKL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for CrkL [p Tyr207] Antibody

• CrkL
• crk-like protein
• pCRKL
• v-crk avian sarcoma virus CT10 oncogene homolog-like
• v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRKL