Crk Antibody

Images

 
Western Blot: Crk Antibody [NBP1-32044] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3].
Immunocytochemistry/ Immunofluorescence: Crk Antibody [NBP1-32044] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Western Blot: Crk Antibody [NBP1-32044] - (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE diluted at 1:1500
Western Blot: Crk Antibody [NBP1-32044] - Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Crk Antibody [NBP1-32044] - Sample(30 ug of whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
 

Independent Antibodies

       

Order Details

Crk Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.
Localization
Cytoplasm, Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Publications
Read Publication using
NBP1-32044 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Crk Antibody

  • adapter molecule crk
  • avian sarcoma virus CT10 (v-crk) oncogene homolog
  • Crk
  • CRKII
  • p38
  • Proto-oncogene c-Crk
  • v-crk avian sarcoma virus CT10 oncogene homolog
  • v-crk sarcoma virus CT10 oncogene homolog (avian)

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Crk Antibody (NBP1-32044)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Crk Antibody (NBP1-32044) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol CRK
Entrez