Crk Antibody - BSA Free

Western Blot: Crk Antibody [NBP1-32044] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3].

Immunocytochemistry/Immunofluorescence: Crk Antibody [NBP1-32044] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Western Blot: Crk Antibody [NBP1-32044] - Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Crk Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Crk Antibody - BSA Free (NBP1-32044) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-Crk Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Crk. The exact sequence is proprietary.
• Localization: Cytoplasm , Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Crk Antibody - BSA Free

• adapter molecule crk
• avian sarcoma virus CT10 (v-crk) oncogene homolog
• Crk
• CRKII
• p38
• Proto-oncogene c-Crk
• v-crk avian sarcoma virus CT10 oncogene homolog
• v-crk sarcoma virus CT10 oncogene homolog (avian)

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Crk Antibody (NBP1-32044)(1)

Publication using NBP1-32044

• Park JO, Choi DY, Choi DS et al. Identification and characterization of proteins isolated from microvesicles derived from Human lung cancer pleural effusions. Proteomics 2013-04-14 [PMID: 23585444] (WB, Human)

Bioinformatics

• Gene Symbol: CRK