CREM Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC215521 and based on accession number NM_182771. The protein contains a C-terminal DDK tag.
Homo sapiens cAMP responsive element modulator (CREM), transcript variant 15, mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for CREM
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for CREM Overexpression Lysate (Native)
- cAMP response element modulator
- cAMP responsive element modulator
- cAMP-responsive element modulator
- hCREM 2alpha-b protein
- hCREM 2beta-a protein
- inducible cAMP early repressor ICER
- Inducible cAMP early repressor
This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq]. Transcript Variant: This variant (15), also known as CREM theta1-tau2-beta, uses an alternative downstream promoter, and differs in the 5' UTR and 5' coding region, compared to variant 1. This results in a shorter isoform (o) with a distinct N-terminus, compared to isoform a. This isoform represents an activator isoform theta 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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