CREM Antibody

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Western Blot: CREM Antibody [NB100-1470] - Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CREM Antibody Summary

Immunogen
Peptide with sequence LETLKDICSPKTDY corresponding to C-Terminus according to NP_853549.1, NP_874387.1, NP_874388.1, NP_874393.1, NP_874394.1, NP_877570.1, NP_877571.1, NP_877572.1, NP_877573.1, NP_878270.1, NP_898883.1.
Specificity
This antibody is expected to recognise the following protein isoforms of human CREM protein: a (NP_853549.1); e (NP_874387.1); f (NP_874388.1); k (NP_874393.1); l (NP_874394.1); m (NP_877570.1); n (NP_877571.1); o (NP_877572.1); p (NP_877573.1); q (NP_878270.1); v (NP_898883.1). This antibody is not expected to cross-react with CREB protein.
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CREM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human heart lysates (calculated MW of 32.5 kDa band according to NP_853549.1).
Control
CREM Overexpression Lysate
Publications
Read Publication using NB100-1470.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CREM Antibody

  • cAMP response element modulator
  • cAMP responsive element modulator
  • cAMP-responsive element modulator
  • hCREM 2alpha-b protein
  • hCREM 2beta-a protein
  • hCREM-2
  • ICER
  • inducible cAMP early repressor ICER
  • Inducible cAMP early repressor
  • MGC111110
  • MGC17881
  • MGC41893

Background

This antibody recognises a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CREM Antibody (NB100-1470)(1)

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CREM
Entrez
Uniprot