CREB [p Ser133] Antibody


Western Blot: CREB [p Ser133] Antibody [NB300-273] - Western blot of forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left lane). Phosphospecificity more
Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was more

Product Details

Reactivity Mu, Rt, Hu, Bv, Ca, Ch, Pm, Sh, Xp, ZeSpecies Glossary
Applications WB, IHC, IHC-Fr

Order Details

CREB [p Ser133] Antibody Summary

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH
p Ser133
Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.
Predicted Species
Human (100%), Sheep (100%), Canine (100%), Chicken (100%), Primate (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000
Application Notes
This antibody has not been validated for use in paraffin embedded sections (IHC-P). For IHC-Fr, the antibody was used at 1:1000 and incubated for 48 hours at 4C. The dilution buffer used was 0.1M PBS, 0.5% Triton-100, and 3% normal serum. No antigen retrieval step was performed.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB300-273 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21838783).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
No Preservative
Immunogen affinity purified

Alternate Names for CREB [p Ser133] Antibody

  • active transcription factor CREB
  • cAMP responsive element binding protein 1
  • cAMP-response element-binding protein-1
  • cAMP-responsive element-binding protein 1
  • CREB
  • CREB1
  • CREB-1
  • cyclic AMP-responsive element-binding protein 1
  • MGC9284
  • pCREB
  • transactivator protein


It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogenactivated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs)phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CREB Antibody (NB300-273)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: WB.

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FAQs for CREB Antibody (NB300-273). (Showing 1 - 1 of 1 FAQs).

  1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
    • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

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Gene Symbol CREB1