Creatine kinase MT 1A Antibody - BSA Free

Immunoprecipitation: Creatine kinase MT 1A Antibody - BSA Free [NBP3-47596] - IP Result of anti-Creatine kinase MT 1A (IP:, 4ug; Detection: 1:1000) with mouse heart tissue lysate 3200ug.

Western Blot: Creatine kinase MT 1A Antibody - BSA Free [NBP3-47596] - Rat heart tissue were subjected to SDS PAGE followed by western blot with (Creatine kinase MT 1A Antibody) at dilution of 1:1500

Immunohistochemistry: Creatine kinase MT 1A Antibody - BSA Free [NBP3-47596] - Immunohistochemistry of paraffin-embedded human breast cancer using (Creatine kinase MT 1A antibody) at dilution of 1:50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Creatine kinase MT 1A Antibody - BSA Free Summary

• Immunogen: creatine kinase, mitochondrial 1A. (Uniprot# P12532)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKMT1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Creatine kinase MT 1A Antibody - BSA Free

• Acidic-Type Mitochondrial Creatine Kinase
• CKMT
• CKMT1
• CKMT1A
• Creatine Kinase U-Type, Mitochondrial
• Creatine Kinase, Mitochondrial 1 (Ubiquitous)
• Creatine Kinase, Mitochondrial 1A
• EC 2.7.3.2
• Mia-CK
• Ubiquitous Mitochondrial Creatine Kinase
• U-MtCK

Background

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CKMT1A