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Creatine kinase MT 1B Antibody

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Western Blot: Creatine kinase MT 1B Antibody [NBP3-05655] - Analysis of extracts of various cell lines, using Creatine kinase MT 1B antibody (NBP3-05655) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western Blot: Creatine kinase MT 1B Antibody [NBP3-05655] - Analysis of extracts of various cell lines, using Creatine kinase MT 1B antibody (NBP3-05655) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: Creatine kinase MT 1B Antibody [NBP3-05655] - Immunofluorescence analysis of U-2 OS cells using Creatine kinase MT 1B Polyclonal Antibody (NBP3-05655) at dilution of 1:100 (40x ...read more
Immunocytochemistry/ Immunofluorescence: Creatine kinase MT 1B Antibody [NBP3-05655] - Immunofluorescence analysis of NIH-3T3 cells using Creatine kinase MT 1B Polyclonal Antibody (NBP3-05655) at dilution of 1:100 (40x ...read more
Immunoprecipitation: Creatine kinase MT 1B Antibody [NBP3-05655] - Analysis of 600ug extracts of Mouse brain cells using 3ug CKMT1B antibody. Western blot was performed from the immunoprecipitate using CKMT1B antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Creatine kinase MT 1B Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human Creatine kinase MT 1B (NP_066270.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CKMT1B
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:50 - 1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Creatine kinase MT 1B Antibody

  • Acidic-Type Mitochondrial Creatine Kinase
  • CKMT
  • CKMT1
  • CKMT1B
  • Creatine Kinase U-Type, Mitochondrial
  • Creatine Kinase, Mitochondrial 1 (Ubiquitous)
  • Creatine Kinase, Mitochondrial 1B
  • EC 2.7.3.2
  • Mia-CK
  • Ubiquitous Mitochondrial Creatine Kinase
  • UMTCK
  • U-MtCK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human cervix shows moderate to strong positivity in mucus in glandular cells.
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Western Blot: DHRS4 Antibody [NBP2-98675] - DHRS4 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate 4 ul anti-DHRS4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DHRS4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa
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Bioinformatics

Gene Symbol CKMT1B