Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free

Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776] - Western blot of mouse mitochondria from whole brain lysateshowing specific immunolabeling of the ~46 kDa CK1Mt proteinphosphorylated at Tyr153 in the first lane (-). Phosphospecificityis shown in the second lane (+) where the immunolabeling isgreatly decreased by blot treatment with lambda phosphatase(Lambda-Ptase, 1200 units for 30 minutes).

• Reactivity: Hu, Mu, Rt, Bv, Ca, Gp, Ha, Pm, Rb, Sh
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr153 conjugated to KLH. Accession # P12532
• Modification: p Tyr153
• Specificity: Specific for endogenous levels of the ~46 kDa Creatine kinase MT 1A protein phosphorylated at Tyr153. Immunolabeling of Creatine kinase MT 1A is greatly decreased by lambda-phosphatase treatment.
• Predicted Species: Rat (100%), Hamster (100%), Canine (100%), Sheep (100%), Primate (100%), Guinea Pig (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKMT1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody detects both Isoform 1 and 2 of the protein.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free

• Acidic-Type Mitochondrial Creatine Kinase
• CKMT
• CKMT1
• CKMT1A
• Creatine Kinase U-Type, Mitochondrial
• Creatine Kinase, Mitochondrial 1 (Ubiquitous)
• Creatine Kinase, Mitochondrial 1A
• EC 2.7.3.2
• Mia-CK
• Ubiquitous Mitochondrial Creatine Kinase
• U-MtCK

Background

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to thecytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes,sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two differentoligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes.Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; thismay be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrialcreatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes locatednear each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CKMT1A