Creatine kinase MT 1A Antibody - BSA Free

Western Blot: Creatine kinase MT 1A Antibody [NBP2-13840] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: Creatine kinase MT 1A Antibody [NBP2-13840] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Creatine kinase MT 1A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Creatine kinase MT 1A Antibody - BSA Free (NBP2-13840) is a polyclonal antibody validated for use in IHC and WB. Anti-Creatine kinase MT 1A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YLIDCERRLERGQDIRIPTPVIHTKH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKMT1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Creatine kinase MT 1A Protein (NBP2-13840PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Creatine kinase MT 1A Antibody - BSA Free

• Acidic-Type Mitochondrial Creatine Kinase
• CKMT
• CKMT1
• CKMT1A
• Creatine Kinase U-Type, Mitochondrial
• Creatine Kinase, Mitochondrial 1 (Ubiquitous)
• Creatine Kinase, Mitochondrial 1A
• EC 2.7.3.2
• Mia-CK
• Ubiquitous Mitochondrial Creatine Kinase
• U-MtCK

Background

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to thecytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes,sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two differentoligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes.Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; thismay be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrialcreatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes locatednear each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Creatine kinase MT 1A Antibody (NBP2-13840)(1)

Publication using NBP2-13840

• Pavon MA, Parreno M, Tellez-Gabriel M et al. CKMT1 and NCOA1 expression as a predictor of clinical outcome in patients with advanced-stage head and neck squamous cell carcinoma. Head Neck. 2015-10-30 [PMID: 26516695] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CKMT1A