Creatine Kinase BB Antibody (8E4) - BSA Free

Western Blot: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Lane 1: HeLa cell lysates, Lane 2: 293T cell lysates, Lane 2: K562 cell lysates, Lane 2: LnCap cell lysates

Immunocytochemistry/Immunofluorescence: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Analysis of CKB in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 8E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Creatine Kinase BB Antibody (8E4) - BSA Free Summary

• Description: Novus Biologicals Mouse Creatine Kinase BB Antibody (8E4) - BSA Free (NBP2-59462) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Creatine Kinase BB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Creatine Kinase BB (1-381aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CKB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1063
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Creatine Kinase BB Antibody (8E4) - BSA Free

• B-CK
• CKB
• Creatine Kinase BB
• Creatine Kinase Isoenzyme

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Creatine Kinase BB Antibody (NBP2-59462)(1)

Publication using NBP2-59462

• Wu K, Yan M, Liu T et al. Creatine kinase B suppresses ferroptosis by phosphorylating GPX4 through a moonlighting function Nature cell biology 2023-05-01 [PMID: 37156912]

Bioinformatics

• Gene Symbol: CKB