CRAT Overexpression Lysate (Native)


Western Blot: CRAT Overexpression Lysate (Adult Normal) [NBL1-09460] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRAT.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

CRAT Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC212196 and based on accession number NM_000755. The protein contains a C-terminal DDK tag.
Homo sapiens carnitine acetyltransferase (CRAT), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
69.6 kDa

Lysate Details for CRAT

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CRAT Overexpression Lysate (Native)

  • Carnitine acetylase
  • Carnitine acetyltransferaseCrAT
  • carnitine O-acetyltransferase
  • CAT
  • CAT1EC
  • EC 2.3.1


Carnitine acetyltransferase (CRAT) is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Different subcellular localizations of the CRAT mRNAs are thought to result from alternative splicing of the CRAT gene suggested by the divergent sequences in the 5' region of peroxisomal and mitochondrial CRAT cDNAs and the location of an intron where the sequences diverge. The alternatively splicing of this gene results in three distinct isoforms, one of which contains an N-terminial mitochondrial transit peptide, and has been shown to be located in mitochondria. [provided by RefSeq]. Transcript Variant: This variant (1) is also known as the mitochondrial transcript variant. It encodes the longest isoform (1) that contains a mitochondrial leader peptide.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
Species: Hu, Ye
Applications: WB
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye, Am(-), Av(-), Pm(-)
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Fe, GP, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, GS
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Av, Ch, GP, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-WhMt
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr
Species: Hu, Mu, Bv, Gt, Sh
Applications: WB
Species: Hu
Applications: WB, ICC/IF, IHC
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P

Publications for CRAT Lysate (NBL1-09460) (0)

There are no publications for CRAT Lysate (NBL1-09460).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CRAT Lysate (NBL1-09460) (0)

There are no reviews for CRAT Lysate (NBL1-09460). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CRAT Lysate (NBL1-09460) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CRAT Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for CRAT Lysate (NBL1-09460)

Discover related pathways, diseases and genes to CRAT Lysate (NBL1-09460). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CRAT Lysate (NBL1-09460)

Discover more about diseases related to CRAT Lysate (NBL1-09460).

Pathways for CRAT Lysate (NBL1-09460)

View related products by pathway.

PTMs for CRAT Lysate (NBL1-09460)

Learn more about PTMs related to CRAT Lysate (NBL1-09460).

Research Areas for CRAT Lysate (NBL1-09460)

Find related products by research area.

Blogs on CRAT.

FANCD2 and DNA damage repair
Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CRAT

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-09460 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

CRAT Recombinant Protein
CRAT Antibody
CRAT Antibody
Albumin Antibody