CRAT Antibody

Western Blot: CRAT Antibody [NBP2-15999] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Discontinued

CRAT Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CAT1. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum; Peroxisome; Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
• Predicted Species: Rat (91%), Porcine (94%), Bovine (96%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRAT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29350465).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for CRAT Antibody

• Carnitine acetylase
• Carnitine acetyltransferaseCrAT
• carnitine O-acetyltransferase
• CAT
• CAT1EC 2.3.1.7
• EC 2.3.1

Background

Carnitine acetyltransferase (CRAT) is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Different subcellular localizations of the CRAT mRNAs are thought to result from alternative splicing of the CRAT gene suggested by the divergent sequences in the 5' region of peroxisomal and mitochondrial CRAT cDNAs and the location of an intron where the sequences diverge. The alternatively splicing of this gene results in three distinct isoforms, one of which contains an N-terminial mitochondrial transit peptide, and has been shown to be located in mitochondria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CRAT Antibody (NBP2-15999)(2)

Publications using NBP2-15999

• Wallace M, Green CR, Roberts LS et al. Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues. Nat. Chem. Biol. 2018-11-01 [PMID: 30327559] (WB, Mouse)
• Schonke M, Bjornholm M, Chibalin AV et al. Proteomics Analysis of Skeletal Muscle from Leptin-Deficient Ob/Ob Mice Reveals Adaptive Remodeling of Metabolic Characteristics and Fiber Type Composition Proteomics 2018-01-19 [PMID: 29350465] (Mouse)

Bioinformatics

• Gene Symbol: CRAT
• Uniprot:
  • P43155(Human)