CRAT Antibody

Western Blot: CRAT Antibody [NBP1-32108] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CAT1 antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: CRAT Antibody [NBP1-32108] - Methanol-fixed H1299, using CRAT (NBP1-32108) antibody at 1:500 dilution.

Western Blot: CRAT Antibody [NBP1-32108] - Sample(30 ug of whole cell lysate)A:Raji 10% SDS PAGE, antibody diluted at 1:1500.

Product Discontinued

CRAT Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CAT1. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum, Peroxisome, Mitochondrion inner membrane, Peripheral membrane protein, Matrix side, Endoplasmic reticulum, Peroxisome, Mitochondrion inner membrane
• Predicted Species: Mouse (90%), Porcine (92%), Bovine (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRAT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for CRAT Antibody

• Carnitine acetylase
• Carnitine acetyltransferaseCrAT
• carnitine O-acetyltransferase
• CAT
• CAT1EC 2.3.1.7
• EC 2.3.1

Background

Carnitine acetyltransferase (CRAT) is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Different subcellular localizations of the CRAT mRNAs are thought to result from alternative splicing of the CRAT gene suggested by the divergent sequences in the 5' region of peroxisomal and mitochondrial CRAT cDNAs and the location of an intron where the sequences diverge. The alternatively splicing of this gene results in three distinct isoforms, one of which contains an N-terminial mitochondrial transit peptide, and has been shown to be located in mitochondria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CRAT Antibody (NBP1-32108)(1)

Publication using NBP1-32108

• Wang X, Yang X, Hou Z et al. Whole-brain mapping of metabolic alterations in a mouse model of Alzheimer's disease by desorption electrospray ionization mass spectrometry imaging Talanta 2022-10-01 (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: CRAT
• Entrez:
  • 1384(Human)
• Uniprot:
  • P43155(Human)