![Immunohistochemistry-Paraffin: RDH5 Antibody [NBP1-52047] - (5ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nbp1-52047_goat-polyclonal-rdh5-antibody-imgenex-img-31074-18720231134297.jpg "Immunohistochemistry-Paraffin: RDH5 Antibody [NBP1-52047] - (5ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Description | Novus Biologicals Goat RDH5 Antibody (NBP1-52047) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-RDH5 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Peptide with sequence C-RDRQSLPASNAFV corresponding to internal region according to NP_002896.2. |
| Epitope | C-RDRQSLPASNAFV |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RDH5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Preliminary testing showed a band at approx. 32kDa in Human Heart and approx. 35kDa in Human Breast lysate, after 0.3ug/ml antibody staining. (calculated MW of 35.0kDa according to NP_002896.2). Primary incubation 1 hour at room temperature. IHC-P: Human Retina shows staining of the ganglion cell layer. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for RDH5 Antibody (NBP1-52047)Find related products by research area.
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| Gene Symbol | RDH5 |
| Entrez | |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: RDH5 Antibody [NBP1-52047] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong Endoplasmic Reticulum and weak cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-52047_goat-polyclonal-rdh5-antibody-imgenex-img-31074-187202311342932.jpg "Immunocytochemistry/Immunofluorescence: RDH5 Antibody [NBP1-52047] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong Endoplasmic Reticulum and weak cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Flow Cytometry: RDH5 Antibody [NBP1-52047] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp1-52047_goat-polyclonal-rdh5-antibody-imgenex-img-31074-187202311342920.jpg "Flow Cytometry: RDH5 Antibody [NBP1-52047] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
