CPVL Overexpression Lysate (Native)

Images

 
Western Blot: CPVL Overexpression Lysate (Adult Normal) [NBL1-09452] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPVL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CPVL Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202336 and based on accession number NM_031311. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens carboxypeptidase, vitellogenic-like (CPVL), transcript variant 1, mRNA.
Gene
CPVL

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CPVL

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CPVL Overexpression Lysate (Native)

  • carboxypeptidase, vitellogenic-likeVitellogenic carboxypeptidase-like protein
  • EC 3.4.16
  • EC 3.4.16.-
  • HVLP
  • MGC10029
  • probable serine carboxypeptidase CPVL
  • VCP-like protein
  • VLP

Background

The protein encoded by this gene is a carboxypeptidase and bears strong sequence similarity to serine carboxypeptidases. Carboxypeptidases are a large class of proteases that act to cleave a single amino acid from the carboxy termini of proteins or peptides. The exact function of this protein, however, has not been determined. At least two alternatively spliced transcripts which encode the same protein have been observed. [provided by RefSeq]. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-88582
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-83192
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80104
Species: Hu
Applications: WB
NBP1-87466
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
DDX0390P-100
Species: Mu
Applications: B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vivo
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB110-68136
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB200-112
Species: Hu, Mu, Rt, Ha, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KO
NBP1-81858
Species: Hu
Applications: WB, IHC, IHC-P
NBL1-09452
Species: Hu
Applications: WB

Publications for CPVL Lysate (NBL1-09452) (0)

There are no publications for CPVL Lysate (NBL1-09452).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CPVL Lysate (NBL1-09452) (0)

There are no reviews for CPVL Lysate (NBL1-09452). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CPVL Lysate (NBL1-09452) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CPVL Products

Bioinformatics Tool for CPVL Lysate (NBL1-09452)

Discover related pathways, diseases and genes to CPVL Lysate (NBL1-09452). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CPVL Lysate (NBL1-09452)

Discover more about diseases related to CPVL Lysate (NBL1-09452).
 

Pathways for CPVL Lysate (NBL1-09452)

View related products by pathway.

PTMs for CPVL Lysate (NBL1-09452)

Learn more about PTMs related to CPVL Lysate (NBL1-09452).

Blogs on CPVL

There are no specific blogs for CPVL, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CPVL Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CPVL