CPT1A Antibody (1081618)

CPT1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CPT1A Monoclonal Antibody (Catalog # MAB11570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 1081618
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CPT1A Antibody (1081618) Summary

• Specificity: Detects a synthetic peptide corresponding to residues surrounding amino acid 501 of human CPT1A protein in direct ELISA.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CPT1A Antibody (1081618)

• carnitine O-palmitoyltransferase 1, liver isoform
• Carnitine O-palmitoyltransferase I, liver isoform
• carnitine palmitoyltransferase 1A (liver)
• Carnitine palmitoyltransferase 1A
• carnitine palmitoyltransferase I, liver
• CPT I
• CPT1
• CPT1A
• CPT1-L
• CPT1-LEC 2.3.1.21
• CPTI-L
• EC 2.3.1
• EC 2.3.1.21
• L-CPT1

Background

CPT1A is one of the isoforms of CPT1.  CPT1 is a mitochondrial enzyme responsible for the formation of acyl carnitines. CPT1A is the rate-limiting enzyme in the fatty acid beta -oxidation, allowing fatty acids to enter the mitochondrial matrix for oxidation. Deficiency or abnormal regulation in this process can result in diseases like metabolic disorders and cancer including but not limited to: CPT1A deficiency, metabolic syndrome, obesity, type 2 diabetes, vascular disease, heart failure, non‑alcoholic fatty liver disease, MS, and renal fibrosis. CPT1A is expressed on the outer mitochondrial membrane of most tissues but predominates in lipogenic tissues such as liver. Genetic, physiological, and dietary modulators are all involved in the regulation of CPT1A. 

1. Brown NF, Hill JK, Esser V, Kirkland JL, Corkey BE, Foster DW, McGarry JD. Mouse white adipocytes and 3T3-L1 cells display an anomalous pattern of carnitine palmitoyltransferase (CPT) I isoform expression during differentiation. Inter-tissue and inter-species expression of CPT I and CPT II enzymes. Biochem J. 1997 Oct 1;327 ( Pt 1)(Pt 1):225-31. doi: 10.1042/bj3270225. PMID: 9355756; PMCID: PMC1218784.
2. Liang K. Mitochondrial CPT1A: Insights into structure, function, and basis for drug development. Front Pharmacol. 2023 Mar 23;14:1160440. doi: 10.3389/fphar.2023.1160440. PMID: 37033619; PMCID: PMC10076611.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P50416(Human)