CPSF6 Antibody (3F11) - Azide and BSA Free

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of CPSF6 expression in Raw 264.7 (Cat # L024V1).

Immunocytochemistry/Immunofluorescence: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of monoclonal antibody to CPSF6 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of CPSF6 expression in HeLa (Cat # L013V1).

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of CPSF6 expression in HL-60 (Cat # L014V1).

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of CPSF6 expression in transfected 293T cell line by CPSF6 monoclonal antibody (M10), clone 3F11. Lane 1: CPSF6 transfected lysatE (59.21 KDa). Lane 2: Non-transfected lysate.

ELISA: CPSF6 Antibody (3F11) [H00011052-M10] - Detection limit for recombinant GST tagged CPSF6 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 3F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CPSF6 Antibody (3F11) - Azide and BSA Free Summary

• Immunogen: CPSF6 (NP_008938, 37 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEAS
• Specificity: CPSF6 (3F11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CPSF6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CPSF6 Antibody (3F11) - Azide and BSA Free

• CFIM
• CFIm68
• CFIM68CPSF 68 kDa subunit
• CFIMcleavage and polyadenylation specific factor 6, 68kD subunit
• cleavage and polyadenylation specific factor 6, 68kDa
• Cleavage and polyadenylation specificity factor 68 kDa subunit
• cleavage and polyadenylation specificity factor subunit 6
• HPBRII-4
• HPBRII-7
• pre-mRNA cleavage factor I, 68kD subunit
• pre-mRNA cleavage factor Im (68kD)
• Pre-mRNA cleavage factor Im 68 kDa subunit
• Protein HPBRII-4/7

Background

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CPSF6 Antibody (H00011052-M10)(4)

Publications using H00011052-M10

• Selyutina A, Hu P, Miller S et al. GS-CA1 and lenacapavir stabilize the HIV-1 core and modulate the core interaction with cellular factors iScience 2022-01-01 [PMID: 35005542] (ICC/IF, WB, Human)
• Selyutina A, Persaud M, Lee K et al. Nuclear Import of the HIV-1 Core Precedes Reverse Transcription and Uncoating Cell Reports 2020-09-29 [PMID: 32997983]
• Kim S, Yamamoto J, Chen Y et al. Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation. Genes Cells. 2010-07-29 [PMID: 20695905]
• Larriba MJ, Casado-Vela J, Pendas-Franco N et al. Novel snail1 target proteins in human colon cancer identified by proteomic analysis. PLoS One. 2010-04-20 [PMID: 20421926]

Bioinformatics

• Gene Symbol: CPSF6
• Entrez:
  • 11052(Human)
• OMIM:
  • 604979(Human)
• Uniprot:
  • NP_008938(Human)