![Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - ICP4 containing complexes include TFIIH, cleavage and polyadenylation complex and chromatin remodeling complexes. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against CPSF2 and CPSF7 of the cleavage and polyadenylation complex were used. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/pre-mRNA-cleavage-factor-I-59-kDa-subunit-Antibody-Western-Blot-NB100-61600-img0006.jpg "Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - ICP4 containing complexes include TFIIH, cleavage and polyadenylation complex and chromatin remodeling complexes. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against CPSF2 and CPSF7 of the cleavage and polyadenylation complex were used. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY license.")
| Reactivity | Hu, Ma, Pm, Mu, Rt, Ca, Pm, Eq, Gp, Pm-Or, RMSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit pre-mRNA cleavage factor I (59 kDa subunit) Antibody - BSA Free (NB100-61600) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-pre-mRNA cleavage factor I (59 kDa subunit) Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human cleavage and polyadenylation specificity factor 7, 59 kDa subunit using the numbering given in entry NP_079087.2 (GeneID 79869). |
| Predicted Species | Mouse (100%), Rat (100%), Canine (100%), Equine (100%), Rhesus Macaque (100%), Orangutan (100%), Guinea Pig (100%), Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CPSF7 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Although not tested this antibody may be useful in Immunocytochemistry/Immunoflourescence. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CPSF7 |
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![Immunohistochemistry: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CPSF59 used at a dilution of 1:500 (2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/pre-mRNA-cleavage-factor-I-59-kDa-subunit-Antibody-Immunohistochemistry-NB100-61600-img0005.jpg "Immunohistochemistry: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CPSF59 used at a dilution of 1:500 (2ug/ml). Detection: DAB")
![Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CPSF59 antibody NB100-61600 used for IP at 3 mcg/mg lysate. CPSF59 was also immunoprecipitated by rabbit anti-CPSF59 antibody NB100-61599, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/pre-mRNA-cleavage-factor-I-59-kDa-subunit-Antibody-Western-Blot-NB100-61600-img0002.jpg "Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CPSF59 antibody NB100-61600 used for IP at 3 mcg/mg lysate. CPSF59 was also immunoprecipitated by rabbit anti-CPSF59 antibody NB100-61599, which recognizes an upstream epitope.")
![Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CPSF59 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/pre-mRNA-cleavage-factor-I-59-kDa-subunit-Antibody-Immunohistochemistry-Paraffin-NB100-61600-img0001.jpg "Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CPSF59 used at a dilution of 1:250.")
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg)
![Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")