pre-mRNA cleavage factor I (59 kDa subunit) Antibody

Images

 
Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - ICP4 containing complexes include TFIIH, cleavage and polyadenylation complex and chromatin remodeling complexes. Western blot analysis ...read more
Immunohistochemistry: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CPSF59 used at a dilution of 1:500 ...read more
Independent Antibodies: Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CPSF59 used at a dilution of 1:250.

Product Details

Summary
Reactivity Hu, Ma, Pm, Mu, Rt, Ca, Pm, Eq, Gp, Pm-Or, RMSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

pre-mRNA cleavage factor I (59 kDa subunit) Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human cleavage and polyadenylation specificity factor 7, 59 kDa subunit using the numbering given in entry NP_079087.2 (GeneID 79869).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Equine (100%), Rhesus Macaque (100%), Orangutan (100%), Guinea Pig (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPSF7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:100-1:2000
Application Notes
Although not tested this antibody may be useful in Immunocytochemistry/Immunoflourescence.
Control
pre-mRNA cleavage factor I (59 kDa subunit) Overexpression Lysate
Publications
Read Publications using
NB100-61600 in the following applications:

  • WB
    2 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with White-tufted-ear marmoset, Crab-eating Macaque, Small-eared Galago and Northern White-cheeked Gibbon. Mammal reactivity reported in scientific literature (PMID: 27180817). Primate reactivity reported in scientific literature (PMID: 24147125).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for pre-mRNA cleavage factor I (59 kDa subunit) Antibody

  • CFIm59
  • cleavage and polyadenylation specific factor 7, 59kDa
  • CPSF 59 kDa subunit
  • FLJ12529,59 kDa subunit
  • FLJ39024
  • MGC9315
  • pre-mRNA cleavage factor I, 59 kDa subunit

Background

CPSF59 is the the 59 kDa subunit of the cleavage factor Im complex (CFIm). The CFIm is a heterodimer composed of a 25 kDa (CPSF5) subunit and either the 59 kDa (CPSF7) or 68 kDa (CPSF6) subunit and is essential to the first step of pre-mRNA 3 prime-end processing. CPSF59 bears an RNA-recognition motif (RRM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for pre-mRNA cleavage factor I (59 kDa subunit) Antibody (NB100-61600)(2)

We have publications tested in 2 confirmed species: Mammal, Monkey.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Mammal
(1)
Monkey
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for pre-mRNA cleavage factor I (59 kDa subunit) Antibody (NB100-61600) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CPSF7
Entrez
Uniprot