CPNE1 Antibody - BSA Free

Immunohistochemistry-Paraffin: CPNE1 Antibody [NBP1-32194] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.

Western Blot: CPNE1 Antibody [NBP1-32194] - Copine I antibody [N3C3] detects Copine I protein by western blot analysis. A. 30 ug 293T whole cell lysate/extractB. 30 ug A431 whole cell lysate/extractC. 30 ug HeLa whole cell lysate/extractD. 30 ug HepG2 whole cell lysate/extract7.5 % SDS-PAGECopine I antibody [N3C3] (NBP1-32194) dilution: 1:500

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CPNE1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CPNE1 Antibody - BSA Free (NBP1-32194) is a polyclonal antibody validated for use in IHC and WB. Anti-CPNE1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CPNE1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPNE1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CPNE1 Antibody - BSA Free

• copine ICPN1COPN1
• copine-1
• MGC1142

Background

Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Sequence analysis identified multiple alternatively spliced variants in the 5' UTR. All variants encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CPNE1 Antibody (NBP1-32194)(1)

Publication using NBP1-32194

• Talaat IM, Abu-Gharbieh E, Hussein A et al. Prognostic Value of Copine 1 in Patients With Renal Cell Carcinoma Anticancer research 2022-01-01 [PMID: 34969745]

Bioinformatics

• Gene Symbol: CPNE1