COX5b Antibody

Images

 
Western Blot: COX5b Antibody [NBP1-31303] - Sample(30 ug of whole cell lysate)A:Hep G2 15% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: COX5b Antibody [NBP1-31303] - Paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.00 mg/ml

Order Details

COX5b Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human COX5B. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COX5B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (85%), Canine (83%), Porcine (83%), Rat (82%), Bovine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1.00 mg/ml
Purity
Protein A purified

Alternate Names for COX5b Antibody

  • COXVB
  • Cytochrome c oxidase polypeptide Vb
  • cytochrome c oxidase polypeptide VB, mitochondrial
  • cytochrome c oxidase subunit 5B, mitochondrial
  • cytochrome c oxidase subunit Vb

Background

Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol COX5B