Corticotropin Releasing Factor Antibody

Product Discontinued

Corticotropin Releasing Factor Antibody Summary

• Immunogen: A synthetic peptide from C-terminus of human CRF protein
• Specificity: This product is specific for Corticotropin Releasing Factor
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot
• Application Notes: IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Use in Western blot reported in scientific literature (PMID 26987580).
• Theoretical MW: 4.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken, Canine, Frog,Porcine, Rat. Mouse reactivity reported in scientific literature (PMID: 26987580)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.6) and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Corticotropin Releasing Factor Antibody

• corticoliberin
• corticotropin releasing hormone
• Corticotropin-releasing hormone
• CRFCorticotropin-releasing factor

Background

CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Corticotropin Releasing Factor Antibody (NBP1-35703)(3)

Publications using NBP1-35703

• Koutmani Y, Gampierakis IA, Polissidis A et al. CRH Promotes the Neurogenic Activity of Neural Stem Cells in the Adult Hippocampus Cell Rep 2019-10-22 [PMID: 31644914]
• Giannogonas P, Apostolou A, Manousopoulou A et al. Identification of a novel interaction between corticotropin releasing hormone (Crh) and macroautophagy. Sci Rep. 2016-03-18 [PMID: 26987580] (IHC-P, WB, Mouse)
• Helmo FR, Machado JR, Oliveira LF et al. Morphological and inflammatory changes in the skin of autopsied fetuses according to the type of stress. Pathol. Res. Pract. 2015-09-05 [PMID: 26384577] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CRH
• Entrez:
  • 1392(Human)