Connexin 43/GJA1 [p Ser368] Antibody

Images

 
Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722] - rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling ...read more

Product Details

Summary
Reactivity Mu, Rt, Hu, Mu, Bv, Ca, Ch, GP, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Connexin 43/GJA1 [p Ser368] Antibody Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in rat brain CaM Kinase II.
Modification
p Ser368
Localization
Cytoplasmic and Integral membrane protein
Marker
Gap Junctions Marker
Specificity
Specific for the ~43k connexin43 protein phosphorylated at Ser368. Immunolabeling is blocked by lambda-phosphatase treatment.
Predicted Species
Human (100%), Mouse (100%), Bovine (100%), Zebrafish (100%), Canine (100%), Guinea Pig (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GJA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~43 kDa.
Positive Control
Connexin 43/GJA1 Lysate (NBL1-11086)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for Connexin 43/GJA1 [p Ser368] Antibody

  • Connexin 43
  • connexin-43
  • CX43
  • CX43gap junction protein, alpha-like
  • DFNB38
  • Gap junction 43 kDa heart protein
  • gap junction alpha-1 protein
  • gap junction protein, alpha 1, 43kDa (connexin 43)
  • gap junction protein, alpha 1, 43kDa
  • GJA1
  • GJAL
  • HSS
  • ODD
  • ODDD
  • ODOD
  • SDTY3

Background

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-46568
Species: Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-53384
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC-P
NBP1-85047
Species: Hu, Po
Applications: Simple Western, ICC/IF, IHC, IHC-P, ICC
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-59254
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P
NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-48309
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-58660
Species: Hu
Applications: IHC-P

Publications for Connexin 43/GJA1 Antibody (NB100-722) (0)

There are no publications for Connexin 43/GJA1 Antibody (NB100-722).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Connexin 43/GJA1 Antibody (NB100-722) (0)

There are no reviews for Connexin 43/GJA1 Antibody (NB100-722). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Connexin 43/GJA1 Antibody (NB100-722) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Connexin 43/GJA1 Products

Bioinformatics Tool for Connexin 43/GJA1 Antibody (NB100-722)

Discover related pathways, diseases and genes to Connexin 43/GJA1 Antibody (NB100-722). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Connexin 43/GJA1 Antibody (NB100-722)

Discover more about diseases related to Connexin 43/GJA1 Antibody (NB100-722).
 

Pathways for Connexin 43/GJA1 Antibody (NB100-722)

View related products by pathway.

PTMs for Connexin 43/GJA1 Antibody (NB100-722)

Learn more about PTMs related to Connexin 43/GJA1 Antibody (NB100-722).
 

Research Areas for Connexin 43/GJA1 Antibody (NB100-722)

Find related products by research area.

Blogs on Connexin 43/GJA1.

Connexin: Bridging the Gap of Intercellular Communication
Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Connexin 43/GJA1 [p Ser368] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GJA1
Entrez
Uniprot