Connexin 30/GJB6 Antibody (CL4540)

Immunocytochemistry/Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141] - Staining of MCF7 cells using the Anti-GJB6 monoclonal antibody, showing no specific staining of cell junctions. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141] - Staining of RT-4 cells using the Anti-GJB6 monoclonal antibody, showing specific staining of cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL4540
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Connexin 30/GJB6 Antibody (CL4540) Summary

• Immunogen: This antibody was developed against a synthetic peptide corresponding to the following amino acid sequence:RSKRAQTQKNHPNHALKESK
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GJB6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC reactivity reported in (PMID: 31205407).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Connexin 30/GJB6 Antibody (CL4540)

• Connexin 30
• connexin-30
• CX30
• CX30gap junction protein, beta 6
• DFNA3B
• DFNB1B
• ectodermal dysplasia 2, hidrotic (Clouston syndrome)
• ED2
• EDH
• gap junction beta-6 protein
• gap junction protein, beta 6 (connexin 30)
• gap junction protein, beta 6, 30kDa
• GJB6
• HEDDFNA3

Background

The connexin gene family codes for the protein subunits of gap junction channels that mediate direct diffusion of ions and metabolites between the cytoplasm of adjacent cells. Connexins span the plasma membrane 4 times, with amino- and carboxy-terminal regions facing the cytoplasm. Connexin genes are expressed in a cell type-specific manner with overlapping specificity. The gap junction channels have unique properties depending on the type of connexins constituting the channel.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Connexin 30/GJB6 Antibody (NBP2-61141)(1)

Publication using NBP2-61141

• Yu H, Miao Y, Chen W, Qi X et al. Expressional and functional involvement of gap junctions in aqueous humor outflow into the ocular trabecular meshwork of the anterior chamber Molecular Vision 2019-04-26 [PMID: 31205407] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: GJB6