Recombinant Human COMT Protein

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SDS-Page: COMT Protein [NBC1-22920] - COMT, 24.4 kDa (221 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human COMT Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 51-271 of Human COMT

Source: E.coli

Amino Acid Sequence: MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
COMT
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0) containing 1 mM MgCl2, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human COMT Protein

  • catechol O-methyltransferase
  • catechol-O-methyltransferase
  • COMT
  • EC 2.1.1.6

Background

Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be located in the postsynaptic neuron and plays roles in the metabolism of catechol estrogens and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT inhibitors prevent levodopa degradation, increase its availability and are used in the treatment of patients with Parkinson's disease. Recombinant human COMT protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for COMT Protein (NBC1-22920) (0)

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FAQs for COMT Protein (NBC1-22920). (Showing 1 - 1 of 1 FAQ).

  1. Is this enzyme (COMT Protein [NBC1-22920] ) active?
    • We have not yet tested this particular protein in functional assays and therefore we are not sure of the activity of the enzyme. We have only validated it for use in ELISA and SDS PAGE gels.

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Bioinformatics

Gene Symbol COMT
Entrez