COMT Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

COMT Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human COMT
  Gly52-Pro271 (Val158Met)
  Accession # P21964
• Specificity: Detects human COMT in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COMT Antibody [Alexa Fluor® 750]

• catechol O-methyltransferase
• catechol-O-methyltransferase
• COMT
• EC 2.1.1.6

Background

COMT (Catechol-O-MethylTransferase) is a 29-30 kDa member of the COMT family of enzymes. It shares the same acronym with plant COMT, or caffeic acid 3-O-methyltransferase that is involved in phenylpropanoid synthesis. Mammalian COMT is widely expressed, and catalyzes the O-methylation of catecholamines, dopamine and estrogen. Human COMT is a 271 amino acid (aa) type II transmembrane (TM) protein. It contains a six aa cytoplasmic N-terminus, an 20 aa TM segment, and a 245 aa luminal/extracellular region that possesses a methyltransferase domain (aa 112-217). This TM form of COMT (termed MB-COMT) is generally associated with pre- and postsynaptic neurons plus glia, and is suggested to be very important in deactivating neurotransmitters, an activity that likely impacts cognition. There is also a 25-26 kDa COMT isoform (termed S-COMT) that utilizes an alternative start site at Met51. This isoform is cytoplasmic and generally found outside the CNS in cells such as mammary and intestinal epithelium, renal tubule epithelium, pancreatic beta -cells, monocytes and mast cells. S-COMT is believed to inactive toxic catechols (hydroxylated benzenes found in plant walls). MB-CORM exhibits high affinity:low capacity activity, while S-COMT shows low affinity:high capacity activity. Two additional alternative start sites have been reported. One starts at Met90, while a second possesses a start site 38 aa upstream of the MB-COMT start site. Over aa 52-271, human COMT shares 79% aa sequence identity with mouse COMT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.