Complement iC3b Antibody (9)

• Reactivity: Hu
• Applications: ELISA, IP
• Clone: 9
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Complement iC3b Antibody (9) Summary

• Description: Novus Biologicals Rat Complement iC3b Antibody (9) (NBP3-11285) is a monoclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The monocolonal antibody 9 (also known as YB2/90-5-20) reacts with a neoantigen on iC3, iC3b, C3dg and C3g.
• Specificity: The monoclonal antibody 9 recognizes iC3b, C3dg and C3g in plasma. The monoclonal antibody does not recognize C3 or C3b.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: C3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunoprecipitation
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Complement iC3b Antibody (9)

• Complement C3dg fragment
• Complement C3g fragment
• Complement fragment iC3b
• iC3b
• Inactivated C3b

Background

The monocolonal antibody 9 (also known as YB2/90-5-20) reacts with a neoantigen on iC3, iC3b, C3dg and C3g. C3g itself however is a small fragment probably not formed in vivo. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes the person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody-mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Proteolysis by C3-convertases results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b and C3f. iC3b is further processed into C3c and C3dg. C3dg can be cleaved into C3d and C3g though this does not occur in plasma. The monoclonal antibody 9 recognizes iC3b, C3dg and C3g in plasma. The monoclonal antibody does not recognize C3 or C3b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C3