Complement Factor H-related 5/CFHR5 Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Factor H-related 5/CFHR5 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Complement Factor H-related 5/CFHR5
  Glu19-Glu569
  Accession # Q9BXR6
• Specificity: Detects human Complement Factor H-related 5/CFHR5 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CFHR5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Factor H-related 5/CFHR5 Antibody

• CFHL5
• CFHL5factor H-related protein 5
• CFHR5
• Complement Factor Hrelated 5
• Complement Factor H-related 5
• FHR5
• FHR-5complement factor H-related protein 5
• FHR5MGC133240
• FLJ10549

Background

The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H‑like protein 1 (FHL-1) and five factor H‑related proteins (FHR-1 to -5) (2). Members of this family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. The genes of this family have been located in human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (3). FHR-5 has been identified initially as a universal component of complement deposits (1), and detected in glomerular immune deposits (4). The pattern of deposits is similar to other complement components, suggesting that FHR-5 may play a role in complement activation and regulation. It is synthesized in the liver and consists of 9 SCRs. Its biological function is not understood fully. FHR-5 exhibits similar characteristics as those of factor H in heparin binding, CRP binding, and lipoprotein association (5). Weak factor I-dependent cofactor activity for C3b cleavage has also been observed (5).

1. McRae, J. L. et al. (2001) J. Biol. Chem. 276:6747.
2. Zipfel, P. F. et al. (2002) Biochem. Soc. Trans. 30:971.
3. McRae, J. L. et al. (2002) Genetica. 114:157.
4. Murphy, B. et al. (2002) Am. J. Kidney. Dis. 39:24.
5. McRae, J. L. et al. (2005) J. Immunol. 174:6250.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Factor H-related 5/CFHR5 Antibody (AF3845)(2)

Publications using AF3845

• Nóra Garam, Marcell Cserhalmi, Zoltán Prohászka, Ágnes Szilágyi, Nóra Veszeli, Edina Szabó et al. FHR-5 Serum Levels and CFHR5 Genetic Variations in Patients With Immune Complex-Mediated Membranoproliferative Glomerulonephritis and C3-Glomerulopathy Frontiers in Immunology 2021-09-10 [PMID: 34566977]
• Csincsi A, Kopp A, Zoldi M, Banlaki Z, Uzonyi B, Hebecker M, Caesar J, Pickering M, Daigo K, Hamakubo T, Lea S, Goicoechea de Jorge E, Jozsi M Factor H-related protein 5 interacts with pentraxin 3 and the extracellular matrix and modulates complement activation. J Immunol, 2015-04-08;194(10):4963-73. 2015-04-08 [PMID: 25855355] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CFHR5
• Uniprot:
  • Q9BXR6()