Complement Factor H-related 3/CFHR3 Antibody - BSA Free

Western Blot: Complement Factor H-related 3/CFHR3 Antibody - BSA Free [NBP2-92515] - Western blot analysis of Rat liver, using Complement Factor H-related 3/CFHR3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Immunocytochemistry/ Immunofluorescence: Complement Factor H-related 3/CFHR3 Antibody - BSA Free [NBP2-92515] - Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of rat liver cells using Complement Factor H-related 3/CFHR3 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: Complement Factor H-related 3/CFHR3 Antibody - BSA Free [NBP2-92515] - Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse liver cells using Complement Factor H-related 3/CFHR3 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Complement Factor H-related 3/CFHR3 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-200 of human CFHR3 (NP_066303.2). AVPCLRKCYFPYLENGYNQNYGRKFVQGNSTEVACHPGYGLPKAQTTVTCTEKGWSPTPRCIRVRTCSKSDIEIENGFISESSSIYILNKEIQYKCKPGYATADGNSSGSITCLQNGWSAQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CFHR3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Complement Factor H-related 3/CFHR3 Antibody - BSA Free

• CFHL3;Complement factor H-related protein 3;DOWN16;FHR3;FHR-3;HLF4
• CFHR3
• Complement Factor H-related 3
• DOWN16
• FHR3
• FHR-3
• HFL4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFHR3