Complement Factor H-related 4/CFHR4 Antibody

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Complement Factor H-related 4/CFHR4 Antibody Summary

• Immunogen: Recombinant FHR4 fragment SCR2-4.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CFHR4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Western Blot
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. WB: A non-reduced and/or reduced sample treatment and SDS-Page was used. The band size is 86 kDa. IA: It can be used as detection antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A purified

Alternate Names for Complement Factor H-related 4/CFHR4 Antibody

• complement factor H-related protein 4
• CFHL4
• CFHR4
• Complement Factor Hrelated 4
• Complement Factor H-related 4
• FHR4
• FHR-4

Background

Polyclonal antibody NBP2-77378 recognizes human complement factor H related protein 4 (FHR4). The complement system is the first line of defense against pathogens and facilitates elimination of apoptotic and damaged cells. Complement factor H (fH) is an abundant plasma protein that negatively regulates the alternative pathway and the C3b amplification loop. The proteins encoded by the fH gene family includes besides fH also five fH-related proteins (FHR1-5), encoded by closely linked but unique genes. Like fH these proteins consist of repeating short consensus repeats (SCRs). fH competes with fB for binding to C3b and C3b(H2O) thereby blocking the formation of the convertase, accelerates decay of C3-convertases and is a cofactor for fI. The function of FHR proteins is less clear. FHRs are produced primarily in the liver and secreted in the plasma. FHR4 binds to the C3d region of C3b. In contrast to fH, the FHR4-C3b interaction promotes complement activation. Two transcripts of the FHR4 gene exist: FHR4B and FHR4B, consisting of respectively 9 and 5 SCRs (86 & 42kDa). Both variants have different binding profiles to C3b. Suggested ligand in complement activation of FHR4 is pentameric CRP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFHR4