Complement Factor D/Adipsin Antibody (D10/4) - BSA Free

Western Blot: Complement Factor D/Adipsin Antibody (D10/4) - BSA Free [NBP2-23532] - Lane 1: Purified Factor D Lane 2: Factor D in human serum Lane 3: SDS-PAGE purified Factor D Lane 4: MW-marker

ELISA: Complement Factor D/Adipsin Antibody (D10/4) - BSA Free [NBP2-23532] - The calibration curve of a sandwich assay for Factor D using NBP2-23532 as the capture antibody and NBP2-23547 as the biotinylated detection antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: D10/4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Complement Factor D/Adipsin Antibody (D10/4) - BSA Free Summary

• Description: Novus Biologicals Mouse Complement Factor D/Adipsin Antibody (D10/4) - BSA Free (NBP2-23532) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human factor D (1)
• Epitope: Epitope specificity differs from that of Clone 01 .
• Specificity: Clone D10/4 is specific for complement factor D in human serum and plasma
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFD
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-23532 can be used as a capture antibody in sandwich ELISA with biotinylated NBP1-05121 as detection antibody. (1) WB: NBP2-23532 can be used for Western blotting following SDS-PAGE under non-reducing conditions. (2)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement Factor D/Adipsin Antibody (D10/4) - BSA Free

• Adipsin
• ADN
• ADNcomplement factor D
• AMBP-1
• C3 convertase activator
• CFD
• complement factor D (adipsin)
• complement factor D preproprotein
• Complement Factor D
• D component of complement (adipsin)
• DF
• EC 3.4.21
• EC 3.4.21.46
• PFD
• Properdin factor DADIPSIN

Background

Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFD