Complement Factor D/Adipsin Antibody (577620)

Western blot shows lysates of mouse adipose tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Complement Factor D/Adipsin Monoclonal Antibody (Catalog # MAB5430) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Complement Factor D/Adipsin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, IP
• Clone: 577620
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Factor D/Adipsin Antibody (577620) Summary

• Immunogen: NS0 cell-derived recombinant mouse Complement Factor D/Adipsin
  Ile26-Ser259
  Accession # P03953
• Specificity: Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Complement Factors B, D,H, I or rhGranzyme A is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CFD
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Factor D/Adipsin Antibody (577620)

• Adipsin
• ADN
• ADNcomplement factor D
• AMBP-1
• C3 convertase activator
• CFD
• complement factor D (adipsin)
• complement factor D preproprotein
• Complement Factor D
• D component of complement (adipsin)
• DF
• EC 3.4.21
• EC 3.4.21.46
• PFD
• Properdin factor DADIPSIN

Background

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Complement Factor D contains one peptidase S1 domain that cleaves an Arg‑Lys bond. There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse Complement Factor D shares 85% and 68% aa identity with rat and human Complement Factor D, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Factor D/Adipsin Antibody (MAB5430)(4)

Publications using MAB5430

• K Mulfaul, E Ozaki, N Fernando, K Brennan, KR Chirco, E Connolly, C Greene, A Maminishki, RG Salomon, M Linetsky, R Natoli, RF Mullins, M Campbell, SL Doyle Toll-like Receptor 2 Facilitates Oxidative Damage-Induced Retinal Degeneration Cell Rep, 2020-02-18;30(7):2209-2224.e5. 2020-02-18 [PMID: 32075760] (Neutralization, Mouse)
• Mitsukuni Yoshida, Akiko Satoh, Jonathan B. Lin, Kathryn F. Mills, Yo Sasaki, Nicholas Rensing et al. Extracellular Vesicle-Contained eNAMPT Delays Aging and Extends Lifespan in Mice Cell Metabolism 2019-08-01 [PMID: 31204283]
• Y Li, W Zou, JR Brestoff, N Rohatgi, X Wu, JP Atkinson, CA Harris, SL Teitelbaum Fat-Produced Adipsin Regulates Inflammatory Arthritis Cell Rep, 2019-06-04;27(10):2809-2816.e3. 2019-06-04 [PMID: 31167128] (Western Blot, Transgenic Mouse)
• J. Harry Sweigard, Hidetaka Matsumoto, Kaylee E. Smith, Leo A. Kim, Eleftherios I. Paschalis, Yoko Okonuki et al. Inhibition of the alternative complement pathway preserves photoreceptors after retinal injury Science Translational Medicine 2015-07-22 [PMID: 26203084]

Bioinformatics

• Gene Symbol: CFD
• Uniprot:
  • P03953()